8AAJ

Crystal structure of the Pyrococcus abyssi RPA (apo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	C 2 2 21
Unit cell lengths	116.526, 169.738, 201.855
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 3.700
R-factor	0.239
Rwork	0.238
R-free	0.25470
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.630
Data reduction software	XDS (VERSION Feb 5, 2021)
Data scaling software	STARANISO (2.3.36)
Phasing software	SHELXD
Refinement software	BUSTER (2.10.4 (3-FEB-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	3.800
High resolution limit [Å]	3.700	3.700
Rmerge	0.082	4.306
Rmeas	0.083	4.390
Rpim	0.016	0.844
Number of reflections	13191	217
<I/σ(I)>	21.4	1
Completeness [%]	60.1	13.3
Redundancy	27.6	26.8
CC(1/2)	1.000	0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1M Tris pH 8.5 1.2M ammonium sulfate