8AAB
S148F mutant of blue-to-red fluorescent timer mRubyFT
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | mRubyFT S148F mutant of blue-to-red fluorescent timer | polymer | 241 | 27098.1 | 1 | UniProt (Q8ISF8) Pfam (PF01353) | Entacmaea quadricolor | GFP-like chromoprotein |
| 2 | B (A) | water | water | 18.0 | 123 | Chemie (HOH) |
Sequence modifications
A: 9 - 226 (UniProt: Q8ISF8)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| His -2 | - | expression tag |
| Met -1 | - | expression tag |
| Arg 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Met 2 | - | expression tag |
| Val 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Lys 5 | - | expression tag |
| Gly 6 | - | expression tag |
| Glu 7 | - | expression tag |
| Glu 8 | - | expression tag |
| Lys 17 | Met 12 | engineered mutation |
| His 27 | Tyr 22 | engineered mutation |
| Glu 36 | Asp 31 | engineered mutation |
| Ile 51 | Val 46 | engineered mutation |
| Leu 68 | Met 63 | engineered mutation |
| Arg 72 | Lys 67 | engineered mutation |
| Tyr 77 | His 72 | engineered mutation |
| Pro 78 | Thr 73 | engineered mutation |
| Val 107 | Phe 102 | engineered mutation |
| Val 124 | Ala 119 | engineered mutation |
| Gln 125 | Lys 120 | engineered mutation |
| Arg 127 | Thr 122 | engineered mutation |
| Asp 130 | Asn 125 | engineered mutation |
| Pro 136 | Ala 131 | engineered mutation |
| Phe 148 | Asn 143 | engineered mutation |
| Met 152 | Leu 147 | engineered mutation |
| Thr 163 | Ser 158 | engineered mutation |
| His 164 | Gln 159 | engineered mutation |
| Lys 168 | Asn 163 | engineered mutation |
| His 174 | Tyr 169 | engineered mutation |
| Val 180 | Glu 175 | engineered mutation |
| Gly 190 | Glu 185 | engineered mutation |
| Ile 192 | Phe 187 | engineered mutation |
| Ile 197 | Phe 192 | engineered mutation |
| Ala 199 | Phe 194 | engineered mutation |
| Asn 212 | Lys 207 | engineered mutation |
| Leu 218 | Gln 213 | engineered mutation |
| Arg 219 | His 214 | engineered mutation |
| Ser 222 | Ala 217 | engineered mutation |
| Ala 227 | - | expression tag |
| Gly 228 | - | expression tag |
| Arg 229 | - | expression tag |
| Gly 230 | - | expression tag |
| Gly 231 | - | expression tag |
| Met 232 | - | expression tag |
| Asp 233 | - | expression tag |
| Glu 234 | - | expression tag |
| Leu 235 | - | expression tag |
| Tyr 236 | - | expression tag |
| Lys 237 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27098.1 | |
| All* | Total formula weight | 27098.1 |
