8AAB
S148F mutant of blue-to-red fluorescent timer mRubyFT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | KURCHATOV SNC BEAMLINE K4.4 |
Synchrotron site | KURCHATOV SNC |
Beamline | K4.4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-03-27 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.79373 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.524, 66.465, 96.488 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.420 - 1.600 |
R-factor | 0.195 |
Rwork | 0.194 |
R-free | 0.22030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7qgk |
RMSD bond length | 0.011 |
RMSD bond angle | 1.914 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.8) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.420 | 31.420 | 1.580 |
High resolution limit [Å] | 1.550 | 8.490 | 1.550 |
Rmerge | 0.127 | 0.043 | 0.950 |
Rmeas | 0.143 | 0.050 | 1.059 |
Rpim | 0.064 | 0.026 | 0.460 |
Total number of observations | 149362 | 851 | 7516 |
Number of reflections | 30195 | 221 | 1484 |
<I/σ(I)> | 7 | 15.7 | 1.5 |
Completeness [%] | 99.7 | 97.4 | 100 |
Redundancy | 4.9 | 3.9 | 5.1 |
CC(1/2) | 0.995 | 0.993 | 0.501 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 250 mM MgCl2; 100 mM HEPES 20% PEG 3350, pH 7,5 |