8A8M

Structure of the MAPK p38alpha in complex with its activating MAP2K MKK6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mitogen-activated protein kinase 14	polymer	381	43647.7	1	UniProt (Q16539) Pfam (PF00069)	Homo sapiens (human)	MAP kinase 14,MAPK 14,Cytokine suppressive anti-inflammatory drug-binding protein,CSAID-binding protein,CSBP,MAP kinase MXI2,MAX-interacting protein 2,Mitogen-activated protein kinase p38 alpha,MAP kinase p38 alpha,Stress-activated protein kinase 2a,SAPK2a
2	B (B)	Dual specificity mitogen-activated protein kinase kinase 6	polymer	374	41822.8	1	UniProt (P52564) Pfam (PF00069)	Homo sapiens (human)	MAP kinase kinase 6,MAPKK 6,MAPK/ERK kinase 6,MEK 6,Stress-activated protein kinase kinase 3,SAPK kinase 3,SAPKK-3,SAPKK3
3	C, F (A, B)	PHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER	non-polymer		425.2	2	Chemie (AP2)
4	D, E, G, H (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

A: 1 - 360 (UniProt: Q16539)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag
Val 180	Thr 180	engineered mutation

B: 15 - 334 (UniProt: P52564)

PDB	External Database	Details
Met -3	-	initiating methionine
Gly -2	-	expression tag
Ser -1	-	expression tag
Gln 0	-	expression tag
Leu 1	-	expression tag
Leu 2	-	expression tag
Glu 3	-	expression tag
Arg 4	-	expression tag
Arg 5	-	expression tag
Gly 6	-	expression tag
Val 7	-	expression tag
Ser 8	-	expression tag
Glu 9	-	expression tag
Leu 10	-	expression tag
Pro 11	-	expression tag
Pro 12	-	expression tag
Leu 13	-	expression tag
Tyr 14	-	expression tag
Asp 207	Ser 207	engineered mutation
Asp 211	Thr 211	engineered mutation
Leu 335	-	expression tag
Glu 336	-	expression tag
Val 337	-	expression tag
Leu 338	-	expression tag
Phe 339	-	expression tag
Gln 340	-	expression tag
Gly 341	-	expression tag
Pro 342	-	expression tag
Trp 343	-	expression tag
Ser 344	-	expression tag
His 345	-	expression tag
Pro 346	-	expression tag
Gln 347	-	expression tag
Phe 348	-	expression tag
Glu 349	-	expression tag
Lys 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Gly 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Gly 359	-	expression tag
Gly 360	-	expression tag
Ser 361	-	expression tag
Ala 362	-	expression tag
Trp 363	-	expression tag
Ser 364	-	expression tag
His 365	-	expression tag
Pro 366	-	expression tag
Gln 367	-	expression tag
Phe 368	-	expression tag
Glu 369	-	expression tag
Lys 370	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	85470.6
Non-Polymers*	Number of molecules	6
	Total formula weight	947.7
All*	Total formula weight	86418.2

*Water molecules are not included.