8A67
Branched Lys48- and Lys63-linked tri-ubiquitin (K48-K63-Ub3) in complex with matured synthetic nanobody NbSL3.3Q (3rd generation)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E | Polyubiquitin-B | polymer | 72 | 8192.4 | 2 | UniProt (J3QS39) Pfam (PF00240) UniProt (by SIFTS) (P0CG47) In PDB | Homo sapiens (human) | |
| 2 | B, C, F, G | Polyubiquitin-B | polymer | 76 | 8632.9 | 4 | UniProt (J3QS39) Pfam (PF00240) UniProt (by SIFTS) (P0CG47) In PDB | Homo sapiens (human) | |
| 3 | D, H | Synthetic Nanobody NbSL3.3Q | polymer | 148 | 15967.9 | 2 | synthetic construct | ||
| 4 | B, F | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 5 | C, H | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 2 | Chemie (IPA) | |||
| 6 | D, H | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 7 | E | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 8 | E, F | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 9 | water | water | 18.0 | 523 | Chemie (HOH) |
Sequence modifications
B, C, F, G: 1 - 76 (UniProt: J3QS39)
| PDB | External Database | Details |
|---|---|---|
| Arg 48 | Lys 48 | engineered mutation |
| Arg 63 | Lys 63 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 82852.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 516.6 | |
| All* | Total formula weight | 83368.6 |
