8A5K

Structural analysis of 1-deoxy-D-xylulose 5-phosphate synthase from Pseudomonas aeruginosa and Klebsiella pneumoniae reveals conformational changes upon cofactor binding

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	1-deoxy-D-xylulose-5-phosphate synthase	polymer	622	67223.4	6	UniProt (B7V7R4) Pfam (PF13292) Pfam (PF02779) Pfam (PF02780) In PDB	Pseudomonas aeruginosa LESB58	1-deoxyxylulose-5-phosphate synthase,DXP synthase,DXPS
2	A, B, C, D, E...	THIAMINE DIPHOSPHATE	non-polymer		425.3	6	Chemie (TPP)
3	A, B, C, D, E...	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
4	F, A, B, C, D	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
5	D	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	1527	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 29 - 234 (UniProt: B7V7R4)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
Met 20	-	expression tag
Glu 21	-	expression tag
Asn 22	-	expression tag
Leu 23	-	expression tag
Tyr 24	-	expression tag
Phe 25	-	expression tag
Gln 26	-	expression tag
Ser 27	-	expression tag
His 28	-	expression tag

A, B, C, D, E, F: 241 - 622 (UniProt: B7V7R4)

PDB	External Database	Details
Gly 235	-	linker
Gly 236	-	linker
Gly 237	-	linker
Gly 238	-	linker
Gly 239	-	linker
Gly 240	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	403340.3
Non-Polymers*	Number of molecules	21
	Total formula weight	3110.4
All*	Total formula weight	406450.7

*Water molecules are not included.