8A49

Endoglycosidase S in complex with IgG1 Fc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	IgG1 Fc	polymer	227	25641.1	2	Pfam (PF07654) UniProt (by SIFTS) (P0DOX5)	Homo sapiens
2	B, C (C, D)	Secreted endoglycosidase EndoS	polymer	906	102372.3	2	UniProt (Q9APG4) Pfam (PF20746) Pfam (PF22382) UniProt (by SIFTS) (Q99Y92)	Streptococcus pyogenes
3	E (E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1463.3	1	In PDB GlyTouCan (G80858MF)
4	F, G, H, I (A, C, D, B)	water	water		18.0	68	Chemie (HOH)

Sequence modifications

C, D: 100 - 995 (UniProt: Q9APG4)

PDB	External Database	Details
Met 99	-	initiating methionine
Ala 233	Asp 233	engineered mutation
Leu 235	Glu 235	engineered mutation
Leu 996	-	expression tag
Leu 997	-	expression tag
Glu 998	-	expression tag
His 999	-	expression tag
His 1000	-	expression tag
His 1001	-	expression tag
His 1002	-	expression tag
His 1003	-	expression tag
His 1004	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	256026.9
Branched	Number of molecules	1
	Total formula weight	1463.3
All*	Total formula weight	257490.2

*Water molecules are not included.