8A3V

Crystal structure of the Vibrio cholerae replicative helicase (VcDnaB) in complex with its loader protein (VcDciA)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Replicative DNA helicase	polymer	474	52937.6	2	UniProt (A0A085R2T8) Pfam (PF00772) Pfam (PF03796)	Vibrio cholerae
2	C, D (C, D)	DUF721 domain-containing protein	polymer	163	18403.2	2	UniProt (A0A0H5ZA06) Pfam (PF05258)	Vibrio cholerae	Protein of uncharacterized function (DUF721)
3	E, G (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
4	F, H (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 1 - 468 (UniProt: A0A085R2T8)

PDB	External Database	Details
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag

C, D: 2 - 157 (UniProt: A0A0H5ZA06)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142681.7
Non-Polymers*	Number of molecules	4
	Total formula weight	903.0
All*	Total formula weight	143584.7

*Water molecules are not included.