8A3V
Crystal structure of the Vibrio cholerae replicative helicase (VcDnaB) in complex with its loader protein (VcDciA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.984 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 186.515, 186.515, 252.839 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.800 - 2.900 |
| R-factor | 0.2798 |
| Rwork | 0.279 |
| R-free | 0.29010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t66 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.720 |
| Data reduction software | XDS (VERSION Mar 17, 2020) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP (Vers 11.7.03; 13.07.2020) |
| Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.260 | 48.260 | 3.120 |
| High resolution limit [Å] | 2.900 | 9.970 | 2.900 |
| Rmerge | 0.196 | 0.035 | 2.360 |
| Rmeas | 0.201 | 0.036 | 2.421 |
| Rpim | 0.046 | 0.009 | 0.541 |
| Number of reflections | 21186 | 1000 | 1000 |
| <I/σ(I)> | 13.4 | 61.3 | 1.6 |
| Completeness [%] | 94.1 | 100 | 71.7 |
| Redundancy | 17.4 | 16.3 | 19.9 |
| CC(1/2) | 0.999 | 1.000 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 291 | 0.1M Sodium Acetate 0.9M Potassium/Sodium Tartrate |
