8A0E

CryoEM structure of DHS-eIF5A1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Deoxyhypusine synthase	polymer	371	41098.5	2	UniProt (P49366) Pfam (PF01916)	Homo sapiens (human)	DHS
2	C, D (C, D)	Deoxyhypusine synthase	polymer	371	41130.5	2	UniProt (P49366) Pfam (PF01916)	Homo sapiens (human)	DHS
3	E (E)	Eukaryotic translation initiation factor 5A	polymer	156	16998.4	1	UniProt (A0A2Y9EFS4) Pfam (PF21485) Pfam (PF01287) UniProt (by SIFTS) (P63241)	Homo sapiens (human)	eIF-5A
4	F, H, J, L (A, B, C, D)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
5	G, I, K (A, B, D)	SPERMIDINE	non-polymer		145.2	3	Chemie (SPD)

Sequence modifications

A, B: 1 - 369 (UniProt: P49366)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 329	Lys 329	engineered mutation

C, D: 1 - 369 (UniProt: P49366)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 329	Lys 329	engineered mutation

E: 1 - 154 (UniProt: A0A2Y9EFS4)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	181456.4
Non-Polymers*	Number of molecules	7
	Total formula weight	3089.4
All*	Total formula weight	184545.8

*Water molecules are not included.