7ZR5

CryoEM structure of HSP90-CDC37-BRAF(V600E)-PP5(closed) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Heat shock protein HSP 90-beta	polymer	749	86223.5	2	UniProt (P08238) Pfam (PF13589) Pfam (PF00183)	Homo sapiens (human)	HSP 90,Heat shock 84 kDa,HSP 84,HSP84
2	C (C)	Hsp90 co-chaperone Cdc37	polymer	396	46853.8	1	UniProt (Q16543) Pfam (PF03234) Pfam (PF08565) Pfam (PF08564)	Homo sapiens (human)	Hsp90 chaperone protein kinase-targeting subunit,p50Cdc37
3	D (K)	Serine/threonine-protein kinase B-raf	polymer	828	90934.5	1	UniProt (P15056) Pfam (PF02196) Pfam (PF00130) Pfam (PF07714)	Homo sapiens (human)	Proto-oncogene B-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1
4	E (P)	Serine/threonine-protein phosphatase 5	polymer	489	56020.4	1	UniProt (P53041) Pfam (PF08321) Pfam (PF00149) Pfam (PF00515)	Homo sapiens (human)	PP5,Protein phosphatase T,PP-T,PPT
5	F, G (A, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)

Sequence modifications

A, B: 1 - 724 (UniProt: P08238)

PDB	External Database	Details
Met -24	-	initiating methionine
Arg -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Gly -12	-	expression tag
Met -11	-	expression tag
Ala -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Ser -1	-	expression tag
Ala 0	-	expression tag

C: 1 - 378 (UniProt: Q16543)

PDB	External Database	Details
Leu 379	-	expression tag
Glu 380	-	expression tag
Val 381	-	expression tag
Leu 382	-	expression tag
Phe 383	-	expression tag
Gln 384	-	expression tag
Gly 385	-	expression tag
Pro 386	-	expression tag
Leu 387	-	expression tag
Glu 388	-	expression tag
His 389	-	expression tag
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag

K: 1 - 766 (UniProt: P15056)

PDB	External Database	Details
Met -61	-	initiating methionine
Gly -60	-	expression tag
Gly -59	-	expression tag
Ser -58	-	expression tag
His -57	-	expression tag
His -56	-	expression tag
His -55	-	expression tag
His -54	-	expression tag
His -53	-	expression tag
His -52	-	expression tag
His -51	-	expression tag
His -50	-	expression tag
Gly -49	-	expression tag
Gly -48	-	expression tag
Ser -47	-	expression tag
Trp -46	-	expression tag
Ser -45	-	expression tag
His -44	-	expression tag
Pro -43	-	expression tag
Gln -42	-	expression tag
Phe -41	-	expression tag
Glu -40	-	expression tag
Lys -39	-	expression tag
Gly -38	-	expression tag
Gly -37	-	expression tag
Gly -36	-	expression tag
Ser -35	-	expression tag
Gly -34	-	expression tag
Gly -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Ser -27	-	expression tag
Trp -26	-	expression tag
Ser -25	-	expression tag
His -24	-	expression tag
Pro -23	-	expression tag
Gln -22	-	expression tag
Phe -21	-	expression tag
Glu -20	-	expression tag
Lys -19	-	expression tag
Gly -18	-	expression tag
Ala -17	-	expression tag
Glu -16	-	expression tag
Thr -15	-	expression tag
Ala -14	-	expression tag
Val -13	-	expression tag
Pro -12	-	expression tag
Asn -11	-	expression tag
Ser -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Ser -1	-	expression tag
Ala 0	-	expression tag
Glu 600	Val 600	variant

P: 17 - 499 (UniProt: P53041)

PDB	External Database	Details
His 500	-	expression tag
His 501	-	expression tag
His 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	366255.6
Non-Polymers*	Number of molecules	2
	Total formula weight	1014.4
All*	Total formula weight	367270.0

*Water molecules are not included.