7ZM8
CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (inhibited by DDM) - membrane arm
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (1) | NADH-ubiquinone oxidoreductase chain 1 | polymer | 378 | 41716.4 | 1 | UniProt (G1DJA6) Pfam (PF00146) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit ND1 of NADH-ubiquinone oxidoreductase (Complex I) |
| 2 | B (2) | NADH dehydrogenase subunit 2 | polymer | 571 | 64129.2 | 1 | UniProt (G1DJ98) Pfam (PF00361) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit ND2 of NADH-ubiquinone oxidoreductase (Complex I) |
| 3 | C (3) | NADH-ubiquinone oxidoreductase chain 3 | polymer | 146 | 16330.0 | 1 | UniProt (G1DJ99) Pfam (PF00507) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit ND3 of NADH-ubiquinone oxidoreductase (Complex I) |
| 4 | D (4) | NADH-ubiquinone oxidoreductase chain 4 | polymer | 542 | 60810.3 | 1 | UniProt (G1DJA7) Pfam (PF00361) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit ND4 of NADH-ubiquinone oxidoreductase (Complex I) |
| 5 | E (5) | NADH-ubiquinone oxidoreductase chain 5 | polymer | 679 | 75872.0 | 1 | UniProt (G1DJA3) Pfam (PF00662) Pfam (PF00361) Pfam (PF06455) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit ND1 of NADH-ubiquinone oxidoreductase (Complex I) |
| 6 | F (6) | NADH-ubiquinone oxidoreductase chain 6 | polymer | 224 | 24819.3 | 1 | UniProt (G1DJ96) Pfam (PF00499) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit ND6 of NADH-ubiquinone oxidoreductase (Complex I) |
| 7 | G (8) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 | polymer | 86 | 10312.8 | 1 | UniProt (G0SAE9) Pfam (PF05676) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB7 of NADH-ubiquinone oxidoreductase (Complex I) |
| 8 | H (9) | Subunit NDUFS5 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 785 | 87142.3 | 1 | UniProt (G0SG48) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFS5 of NADH-ubiquinone oxidoreductase (Complex I) |
| 9 | I (D) | Subunit NDUFA1 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 86 | 9833.4 | 1 | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFA1 of NADH-ubiquinone oxidoreductase (Complex I) | |
| 10 | J (J) | NADH-ubiquinone oxidoreductase-like protein | polymer | 199 | 21612.6 | 1 | UniProt (G0S2B3) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFA11 of NADH-ubiquinone oxidoreductase (Complex I) |
| 11 | K (L) | NADH-ubiquinone oxidoreductase chain 4L | polymer | 89 | 9838.0 | 1 | UniProt (G1DJA2) Pfam (PF00420) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit ND4L of NADH-ubiquinone oxidoreductase (Complex I) |
| 12 | L (Q) | Acyl carrier protein | polymer | 141 | 15593.6 | 1 | UniProt (G0SBG9) Pfam (PF00550) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFAB1 of NADH-ubiquinone oxidoreductase (Complex I) |
| 13 | M (R) | Complex I-B22 | polymer | 99 | 11636.5 | 1 | UniProt (G0SAY0) Pfam (PF05347) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB9 of NADH-ubiquinone oxidoreductase (Complex I) |
| 14 | N (S) | Complex I-ESSS | polymer | 143 | 15847.8 | 1 | UniProt (G0SAN0) Pfam (PF10183) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB11 of NADH-ubiquinone oxidoreductase (Complex I) |
| 15 | O (U) | NADH-ubiquinone oxidoreductase | polymer | 186 | 21517.6 | 1 | UniProt (G0S0R3) Pfam (PF06747) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFA8 of NADH-ubiquinone oxidoreductase (Complex I) |
| 16 | P (W) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 | polymer | 121 | 14039.2 | 1 | UniProt (G0SB83) Pfam (PF06212) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFA13 of NADH-ubiquinone oxidoreductase (Complex I) |
| 17 | Q (X) | NADH-ubiquinone oxidoreductase-like protein | polymer | 191 | 21645.5 | 1 | UniProt (G0S0S8) Pfam (PF10785) Pfam (PF12853) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NUXM of NADH-ubiquinone oxidoreductase (Complex I) |
| 18 | R (a) | NADH dehydrogenase (Ubiquinone)-like protein | polymer | 815 | 89210.1 | 1 | UniProt (G0RXU4) Pfam (PF05821) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB8 of NADH-ubiquinone oxidoreductase (Complex I) |
| 19 | S (b) | Subunit NDUFC2 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 94 | 10823.5 | 1 | UniProt (G0S812) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFC2 of NADH-ubiquinone oxidoreductase (Complex I) |
| 20 | T (c) | Subunit NDUFB3 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 93 | 11153.5 | 1 | UniProt (G0S681) Pfam (PF08122) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB3 of NADH-ubiquinone oxidoreductase (Complex I) |
| 21 | U (d) | Subunit NDUFB10 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 105 | 12514.3 | 1 | UniProt (G0SEZ1) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB10 of NADH-ubiquinone oxidoreductase (Complex I) |
| 22 | V (e) | Subunit NDUFB2 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 46 | 5278.1 | 1 | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB2 of NADH-ubiquinone oxidoreductase (Complex I) | |
| 23 | W (g) | Subunit NDUFA3 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 82 | 9076.5 | 1 | UniProt (G0RZZ2) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFA3 of NADH-ubiquinone oxidoreductase (Complex I) |
| 24 | X (i) | Subunit NDUFB6 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 93 | 10857.3 | 1 | UniProt (G0S569) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB6 of NADH-ubiquinone oxidoreductase (Complex I) |
| 25 | Y (j) | Subunit NDUFB4 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 75 | 8760.3 | 1 | UniProt (G0S5C8) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB4 of NADH-ubiquinone oxidoreductase (Complex I) |
| 26 | Z (n) | Subunit NDUFB5 of NADH-ubiquinone oxidoreductase (Complex I) | polymer | 184 | 20824.3 | 1 | UniProt (G0S086) | Chaetomium thermophilum var. thermophilum DSM 1495 | Subunit NDUFB5 of NADH-ubiquinone oxidoreductase (Complex I) |
| 27 | AA, EB, NA, QA, SA... (1, i, 5, W) | 1,2-Distearoyl-sn-glycerophosphoethanolamine | non-polymer | 748.1 | 7 | Chemie (3PE) | |||
| 28 | BA, JA, OA, PA (1, 2, 5) | 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 790.1 | 4 | Chemie (PC1) | |||
| 29 | CA, CB, DA, DB, FA... (1, a, g, 2, 3...) | DODECYL-BETA-D-MALTOSIDE | non-polymer | 510.6 | 14 | Chemie (LMT) | |||
| 30 | AB, BB, EA, TA, XA (X, 2, D, S) | CARDIOLIPIN | non-polymer | 1464.0 | 5 | Chemie (CDL) | |||
| 31 | WA (Q) | S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate | non-polymer | 568.7 | 1 | Chemie (ZMP) | |||
| 32 | AC, BC, CC, FB, GB... (i, j, n, 1, 2...) | water | water | 18.0 | 608 | Chemie (HOH) |
Sequence modifications
5: 1 - 679 (UniProt: G1DJA3)
9: 1 - 785 (UniProt: G0SG48)
a: 1 - 815 (UniProt: G0RXU4)
n: 53 - 184 (UniProt: G0S086)
| PDB | External Database | Details |
|---|---|---|
| Arg 445 | - | insertion |
| Leu 446 | - | insertion |
| Ala 447 | - | insertion |
| Ile 448 | - | insertion |
| Asp 449 | - | insertion |
| Asn 450 | - | insertion |
| Phe 451 | - | insertion |
| Phe 452 | - | insertion |
| Ser 453 | - | insertion |
| Ala 454 | - | insertion |
| Gln 455 | - | insertion |
| Ala 456 | - | insertion |
| Ile 457 | - | insertion |
| Lys 458 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Val 100 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Val 166 | Ala 166 | conflict |
| Ala 168 | Met 168 | conflict |
| - | Glu 169 | deletion |
| - | Gly 170 | deletion |
| - | Asp 171 | deletion |
| - | Pro 172 | deletion |
| - | Asp 173 | deletion |
| - | Pro 174 | deletion |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Leu 2 | - | insertion |
| Ala 3 | - | insertion |
| Leu 4 | - | insertion |
| Arg 5 | - | insertion |
| Gln 6 | - | insertion |
| Arg 7 | - | insertion |
| Ala 8 | - | insertion |
| Ala 9 | - | insertion |
| Leu 10 | - | insertion |
| Leu 11 | - | insertion |
| Ala 12 | - | insertion |
| Arg 13 | - | insertion |
| Arg 14 | - | insertion |
| Val 15 | - | insertion |
| Arg 16 | - | insertion |
| Pro 17 | - | insertion |
| Thr 18 | - | insertion |
| Val 19 | - | insertion |
| Val 20 | - | insertion |
| Val 21 | - | insertion |
| Pro 22 | - | insertion |
| Arg 23 | - | insertion |
| Asn 24 | - | insertion |
| Ala 25 | - | insertion |
| Arg 26 | - | insertion |
| Thr 27 | - | insertion |
| Tyr 28 | - | insertion |
| Ala 29 | - | insertion |
| Ser 30 | - | insertion |
| Ser 31 | - | insertion |
| His 32 | - | insertion |
| Asp 33 | - | insertion |
| His 34 | - | insertion |
| Asp 35 | - | insertion |
| His 36 | - | insertion |
| His 37 | - | insertion |
| Asp 38 | - | insertion |
| His 39 | - | insertion |
| His 40 | - | insertion |
| His 41 | - | insertion |
| Asp 42 | - | insertion |
| His 43 | - | insertion |
| Gly 44 | - | insertion |
| His 45 | - | insertion |
| Asn 46 | - | insertion |
| Val 47 | - | insertion |
| Glu 48 | - | insertion |
| Glu 49 | - | insertion |
| Pro 50 | - | insertion |
| Leu 51 | - | insertion |
| Gly 52 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 26 |
| Total formula weight | 701194.6 | |
| Non-Polymers* | Number of molecules | 31 |
| Total formula weight | 23434.6 | |
| All* | Total formula weight | 724629.1 |
