7ZLH

Cryo-EM structure of C-mannosyltransferase CeDPY19, in apo state, bound to CMT2-Fab and anti-Fab nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	H	CMT2-Fab heavy chain	polymer	236	25000.7	1		synthetic construct
2	K	Anti-Fab nanobody	polymer	123	13390.6	1		synthetic construct
3	L	CMT2-Fab light chain	polymer	215	23238.8	1		synthetic construct
4	A	C-mannosyltransferase dpy-19	polymer	707	80892.6	1	UniProt (P34413) Pfam (PF10034) In PDB	Caenorhabditis elegans	Protein dumpy-19
5		water	water		18.0	4	Chemie (HOH)

Sequence modifications

A: 1 - 683 (UniProt: P34413)

PDB	External Database	Details
Leu 684	-	expression tag
Glu 685	-	expression tag
Asp 686	-	expression tag
Tyr 687	-	expression tag
Lys 688	-	expression tag
Asp 689	-	expression tag
His 690	-	expression tag
Asp 691	-	expression tag
Gly 692	-	expression tag
Asp 693	-	expression tag
Tyr 694	-	expression tag
Lys 695	-	expression tag
Asp 696	-	expression tag
His 697	-	expression tag
Asp 698	-	expression tag
Ile 699	-	expression tag
Asp 700	-	expression tag
Tyr 701	-	expression tag
Lys 702	-	expression tag
Asp 703	-	expression tag
Asp 704	-	expression tag
Asp 705	-	expression tag
Asp 706	-	expression tag
Lys 707	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142522.7
All*	Total formula weight	142522.7

*Water molecules are not included.