7ZLG
Cryo-EM structure of C-mannosyltransferase CeDPY19, in complex with acceptor peptide and bound to CMT2-Fab and anti-Fab nanobody
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | H | CMT2-Fab heavy chain | polymer | 236 | 25000.7 | 1 | synthetic construct | ||
2 | K | Anti-Fab nanobody | polymer | 123 | 13390.6 | 1 | synthetic construct | ||
3 | L | CMT2-Fab light chain | polymer | 215 | 23238.8 | 1 | UniProt (Q7Z3Y4) Pfam (PF07686) Pfam (PF07654) In PDB | synthetic construct | |
4 | A | C-mannosyltransferase dpy-19 | polymer | 707 | 80892.6 | 1 | UniProt (P34413) Pfam (PF10034) In PDB | Caenorhabditis elegans | Protein dumpy-19 |
5 | P | Synthetic octapeptide WEHI 1886493 | polymer | 7 | 821.9 | 1 | synthetic construct | ||
6 | water | water | 18.0 | 10 | Chemie (HOH) |
Sequence modifications
L: 2 - 215 (UniProt: Q7Z3Y4)
A: 1 - 683 (UniProt: P34413)
PDB | External Database | Details |
---|---|---|
Ser 1 | - | expression tag |
Arg 19 | Thr 40 | conflict |
Ser 29 | Asp 50 | conflict |
Val 30 | Ile 51 | conflict |
Ser 32 | Asn 53 | conflict |
Ala 33 | Tyr 54 | conflict |
Val 34 | Leu 55 | conflict |
Tyr 37 | Phe 58 | conflict |
Leu 47 | Ser 68 | conflict |
Ser 51 | Gly 72 | conflict |
Tyr 56 | Gln 77 | conflict |
Pro 60 | Gln 81 | conflict |
Arg 62 | Lys 83 | conflict |
Arg 67 | Gly 88 | conflict |
Gly 92 | Tyr 113 | conflict |
Ala 93 | Lys 114 | conflict |
Glu 95 | Tyr 116 | conflict |
Ile 97 | Val 118 | conflict |
Val 105 | Leu 126 | conflict |
Ser 124 | Glu 145 | conflict |
PDB | External Database | Details |
---|---|---|
Leu 684 | - | expression tag |
Glu 685 | - | expression tag |
Asp 686 | - | expression tag |
Tyr 687 | - | expression tag |
Lys 688 | - | expression tag |
Asp 689 | - | expression tag |
His 690 | - | expression tag |
Asp 691 | - | expression tag |
Gly 692 | - | expression tag |
Asp 693 | - | expression tag |
Tyr 694 | - | expression tag |
Lys 695 | - | expression tag |
Asp 696 | - | expression tag |
His 697 | - | expression tag |
Asp 698 | - | expression tag |
Ile 699 | - | expression tag |
Asp 700 | - | expression tag |
Tyr 701 | - | expression tag |
Lys 702 | - | expression tag |
Asp 703 | - | expression tag |
Asp 704 | - | expression tag |
Asp 705 | - | expression tag |
Asp 706 | - | expression tag |
Lys 707 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 143344.6 | |
All* | Total formula weight | 143344.6 |