7ZDQ

Cryo-EM structure of Human ACE2 bound to a high-affinity SARS CoV-2 mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Processed angiotensin-converting enzyme 2	polymer	620	71714.3	1	UniProt (Q9BYF1) Pfam (PF01401)	Homo sapiens (human)
2	B (B)	Spike protein S1	polymer	246	27675.9	1	UniProt (P0DTC2) Pfam (PF09408)	Severe acute respiratory syndrome coronavirus 2
3	C, D, E (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A: 19 - 615 (UniProt: Q9BYF1)

PDB	External Database	Details
Gly 616	-	expression tag
Asn 617	-	expression tag
Ser 618	-	expression tag
Gly 619	-	expression tag
Ser 620	-	expression tag
Met 621	-	expression tag
Asp 622	-	expression tag
Tyr 623	-	expression tag
Lys 624	-	expression tag
Asp 625	-	expression tag
Asp 626	-	expression tag
Asp 627	-	expression tag
Asp 628	-	expression tag
Lys 629	-	expression tag
Gly 630	-	expression tag
Ser 631	-	expression tag
Gly 632	-	expression tag
His 633	-	expression tag
His 634	-	expression tag
His 635	-	expression tag
His 636	-	expression tag
His 637	-	expression tag
His 638	-	expression tag

B: 319 - 541 (UniProt: P0DTC2)

PDB	External Database	Details
Asn 477	Ser 477	engineered mutation
His 498	Gln 498	engineered mutation
Gly 542	-	expression tag
Asn 543	-	expression tag
Ser 544	-	expression tag
Gly 545	-	expression tag
Ser 546	-	expression tag
Tyr 547	-	expression tag
Pro 548	-	expression tag
Tyr 549	-	expression tag
Asp 550	-	expression tag
Val 551	-	expression tag
Pro 552	-	expression tag
Asp 553	-	expression tag
Tyr 554	-	expression tag
Ala 555	-	expression tag
Gly 556	-	expression tag
Ser 557	-	expression tag
Gly 558	-	expression tag
His 559	-	expression tag
His 560	-	expression tag
His 561	-	expression tag
His 562	-	expression tag
His 563	-	expression tag
His 564	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99390.2
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	100053.8

*Water molecules are not included.