7Z9C

E.coli gyrase holocomplex with 217 bp DNA and albicidin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	DNA gyrase subunit A	polymer	881	97854.3	2	UniProt (P0AES4) Pfam (PF00521) Pfam (PF03989)	Escherichia coli str. K-12 substr. MG1655
2	B, D (B, D)	DNA gyrase subunit B	polymer	811	90891.7	2	UniProt (P0AES6) Pfam (PF01751) Pfam (PF21249) Pfam (PF18053) Pfam (PF00986) Pfam (PF02518) Pfam (PF00204)	Escherichia coli str. K-12 substr. MG1655	Type IIA topoisomerase subunit GyrB
3	E (E)	DNA (5'-D(P*AP*AP*AP*TP*CP*TP*GP*TP*GP*CP*GP*GP*GP*T)-3')	polymer	14	4335.8	1		Escherichia phage Mu
4	F (F)	DNA (5'-D(P*AP*GP*AP*AP*TP*CP*AP*GP*GP*CP*AP*TP*AP*A)-3')	polymer	14	4321.9	1		Escherichia phage Mu
5	G (G)	DNA (5'-D(*AP*AP*TP*CP*AP*CP*CP*CP*GP*CP*AP*CP*AP*GP*AP*TP*TP*T)-3')	polymer	18	5444.6	1		Escherichia phage Mu
6	H (H)	DNA (5'-D(*GP*AP*TP*TP*TP*TP*AP*TP*GP*CP*CP*TP*GP*AP*TP*TP*CP*T)-3')	polymer	18	5487.6	1		Escherichia phage Mu
7	I, K (B, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	J (C)	albicidin	non-polymer		842.8	1	Chemie (BWH)
9	L, M, N, O (B, C, D, F)	water	water		18.0	10	Chemie (HOH)

Sequence modifications

A, C: 2 - 875 (UniProt: P0AES4)

PDB	External Database	Details
Ser 1	-	expression tag
Leu 876	-	expression tag
Glu 877	-	expression tag
Val 878	-	expression tag
Leu 879	-	expression tag
Phe 880	-	expression tag
Gln 881	-	expression tag

B, D: 2 - 804 (UniProt: P0AES6)

PDB	External Database	Details
Gly 0	-	expression tag
Pro 1	-	expression tag
Glu 805	-	expression tag
Asn 806	-	expression tag
Leu 807	-	expression tag
Tyr 808	-	expression tag
Phe 809	-	expression tag
Gln 810	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	397081.9
Non-Polymers*	Number of molecules	3
	Total formula weight	891.4
All*	Total formula weight	397973.3

*Water molecules are not included.