7Z97

Crystal structure of the F191M variant of Variovorax paradoxus indole monooxygenase (VpIndA1) in complex with 6-bromoindole

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putative dehydrogenase/oxygenase subunit (Flavoprotein)	polymer	433	47308.6	1	UniProt (E6V140) Pfam (PF17885)	Variovorax paradoxus EPS
2	B (A)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
3	C (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
4	D (A)	6-bromo-1H-indole	non-polymer		196.0	1	Chemie (FK1)
5	E (A)	water	water		18.0	247	Chemie (HOH)

Sequence modifications

A: 1 - 412 (UniProt: E6V140)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
Ile -4	-	expression tag
Glu -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
His 0	-	expression tag
Met 191	Phe 191	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47308.6
Non-Polymers*	Number of molecules	3
	Total formula weight	1043.7
All*	Total formula weight	48352.3

*Water molecules are not included.