7Z8Q

Cryo-EM structure of Mycobacterium tuberculosis RNA polymerase core

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (a, b)	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	2	UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Mycobacterium tuberculosis H37Rv	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (c)	DNA-directed RNA polymerase subunit beta	polymer	1174	129602.3	1	UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Mycobacterium tuberculosis H37Rv	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (d)	DNA-directed RNA polymerase subunit beta'	polymer	1319	147438.3	1	UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Mycobacterium tuberculosis H37Rv	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (e)	DNA-directed RNA polymerase subunit omega	polymer	110	11851.1	1	UniProt (P9WGY5) Pfam (PF01192)	Mycobacterium tuberculosis H37Rv	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F, G (d)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	H (d)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

c: 6 - 1178 (UniProt: P9WGY9)

PDB	External Database	Details
Met 5	-	initiating methionine
Val 6	Ile 6	conflict

d: 4 - 1316 (UniProt: P9WGY7)

PDB	External Database	Details
His 1317	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag
His 1322	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	364382.5
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	364537.6

*Water molecules are not included.