7Z6H
Structure of DNA-bound human RAD17-RFC clamp loader and 9-1-1 checkpoint clamp
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Cell cycle checkpoint control protein RAD9A | polymer | 405 | 44260.0 | 1 | UniProt (Q99638) Pfam (PF04139) In PDB | Homo sapiens (human) | hRAD9,DNA repair exonuclease rad9 homolog A |
| 2 | B, K | Cell cycle checkpoint protein RAD1,Cell cycle checkpoint protein RAD17 | polymer | 1021 | 115195.6 | 2 | UniProt (O60671) UniProt (O75943) Pfam (PF02144) Pfam (PF03215) In PDB | Homo sapiens (human) | hRAD1,DNA repair exonuclease rad1 homolog,Rad1-like DNA damage checkpoint protein,hRad17,RF-C/activator 1 homolog |
| 3 | C | Checkpoint protein HUS1 | polymer | 280 | 31731.9 | 1 | UniProt (O60921) Pfam (PF04005) In PDB | Homo sapiens (human) | hHUS1 |
| 4 | D | Replication factor C subunit 4 | polymer | 363 | 39719.6 | 1 | UniProt (P35249) Pfam (PF00004) Pfam (PF21960) Pfam (PF08542) In PDB | Homo sapiens (human) | Activator 1 37 kDa subunit,A1 37 kDa subunit,Activator 1 subunit 4,Replication factor C 37 kDa subunit,RF-C 37 kDa subunit,RFC37 |
| 5 | E | Replication factor C subunit 3 | polymer | 356 | 40614.3 | 1 | UniProt (P40938) Pfam (PF22534) Pfam (PF13177) Pfam (PF21960) In PDB | Homo sapiens (human) | Activator 1 38 kDa subunit,A1 38 kDa subunit,Activator 1 subunit 3,Replication factor C 38 kDa subunit,RF-C 38 kDa subunit,RFC38 |
| 6 | F | Replication factor C subunit 2 | polymer | 354 | 39203.2 | 1 | UniProt (P35250) Pfam (PF00004) Pfam (PF08542) In PDB | Homo sapiens (human) | Activator 1 40 kDa subunit,A1 40 kDa subunit,Activator 1 subunit 2,Replication factor C 40 kDa subunit,RF-C 40 kDa subunit,RFC40 |
| 7 | G | Replication factor C subunit 5 | polymer | 360 | 40688.8 | 1 | UniProt (P40937) Pfam (PF00004) Pfam (PF21960) Pfam (PF08542) In PDB | Homo sapiens (human) | Activator 1 36 kDa subunit,A1 36 kDa subunit,Activator 1 subunit 5,Replication factor C 36 kDa subunit,RF-C 36 kDa subunit,RFC36 |
| 8 | X, Y | Hairpin DNA | polymer | 77 | 23761.2 | 2 | synthetic construct | ||
| 9 | D, E, F, G, K | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 5 | Chemie (AGS) |
Sequence modifications
A: 1 - 391 (UniProt: Q99638)
B, K: 1 - 282 (UniProt: O60671)
B, K: 328 - 1008 (UniProt: O75943)
D: 1 - 363 (UniProt: P35249)
G: 2 - 340 (UniProt: P40937)
| PDB | External Database | Details |
|---|---|---|
| Thr 392 | - | expression tag |
| Ser 393 | - | expression tag |
| Ala 394 | - | expression tag |
| Gly 395 | - | expression tag |
| Ser 396 | - | expression tag |
| Arg 397 | - | expression tag |
| His 398 | - | expression tag |
| His 399 | - | expression tag |
| His 400 | - | expression tag |
| His 401 | - | expression tag |
| His 402 | - | expression tag |
| His 403 | - | expression tag |
| His 404 | - | expression tag |
| His 405 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 283 | - | linker |
| Arg 284 | - | linker |
| Gly 285 | - | linker |
| Ser 286 | - | linker |
| Met 287 | - | linker |
| Ser 288 | - | linker |
| Ala 289 | - | linker |
| Trp 290 | - | linker |
| Ser 291 | - | linker |
| His 292 | - | linker |
| Pro 293 | - | linker |
| Gln 294 | - | linker |
| Phe 295 | - | linker |
| Glu 296 | - | linker |
| Lys 297 | - | linker |
| Gly 298 | - | linker |
| Ser 299 | - | linker |
| Ala 300 | - | linker |
| Gly 301 | - | linker |
| Ser 302 | - | linker |
| Ala 303 | - | linker |
| Ala 304 | - | linker |
| Gly 305 | - | linker |
| Ser 306 | - | linker |
| Gly 307 | - | linker |
| Ala 308 | - | linker |
| Gly 309 | - | linker |
| Trp 310 | - | linker |
| Ser 311 | - | linker |
| His 312 | - | linker |
| Pro 313 | - | linker |
| Gln 314 | - | linker |
| Phe 315 | - | linker |
| Glu 316 | - | linker |
| Lys 317 | - | linker |
| Leu 318 | - | linker |
| Glu 319 | - | linker |
| Val 320 | - | linker |
| Leu 321 | - | linker |
| Phe 322 | - | linker |
| Gln 323 | - | linker |
| Gly 324 | - | linker |
| Pro 325 | - | linker |
| Gly 326 | - | linker |
| His 327 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Leu 1009 | - | expression tag |
| Glu 1010 | - | expression tag |
| Gly 1011 | - | expression tag |
| Ala 1012 | - | expression tag |
| Ser 1013 | - | expression tag |
| His 1014 | - | expression tag |
| His 1015 | - | expression tag |
| His 1016 | - | expression tag |
| His 1017 | - | expression tag |
| His 1018 | - | expression tag |
| His 1019 | - | expression tag |
| His 1020 | - | expression tag |
| His 1021 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 363 | Cys 363 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 514131.5 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 2616.2 | |
| All* | Total formula weight | 516747.7 |
