7Z5A

Crystal structure of the trapped complex of mouse Endonuclease VIII-LIKE 3 (mNEIL3) and hairpin DNA with 5'overhang

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Endonuclease 8-like 3	polymer	303	33579.7	1	UniProt (Q8K203) Pfam (PF06831)	Mus musculus (house mouse)	DNA glycosylase FPG2,DNA glycosylase/AP lyase Neil3,Endonuclease VIII-like 3,Nei-like protein 3
2	B (E)	DNA (5'-D(P*TP*TP*TP*(PED)P*AP*CP*GP*CP*GP*AP*AP*GP*CP*GP*TP*G)-3')	polymer	19	5719.7	1		synthetic construct
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D, E (A, E)	water	water		18.0	123	Chemie (HOH)

Sequence modifications

A: 1 - 282 (UniProt: Q8K203)

PDB	External Database	Details
Pro 46	Leu 46	conflict
His 90	Pro 90	conflict
Gly 114	Ala 114	conflict
Glu 150	Val 150	conflict
Arg 220	Cys 220	conflict
Gly 256	Asp 256	conflict
Asp 283	-	expression tag
Gly 284	-	expression tag
Leu 285	-	expression tag
Glu 286	-	expression tag
Val 287	-	expression tag
Leu 288	-	expression tag
Phe 289	-	expression tag
Gln 290	-	expression tag
Gly 291	-	expression tag
Pro 292	-	expression tag
Gly 293	-	expression tag
Ser 294	-	expression tag
Ser 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	39299.4
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	39364.8

*Water molecules are not included.