7Z4I
SpCas9 bound to 16-nucleotide complementary DNA substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | sgRNA | polymer | 102 | 33005.6 | 1 | synthetic construct | ||
| 2 | B (C) | Target strand of 16-nucleotide complementary DNA substrate | polymer | 36 | 11009.1 | 1 | synthetic construct | ||
| 3 | C (D) | Non-target strand of 16-nucleotide complementary DNA substrate | polymer | 38 | 11734.5 | 1 | synthetic construct | ||
| 4 | D (B) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158588.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 5 | E, F (A) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 214338.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 78.2 | |
| All* | Total formula weight | 214416.2 |
