7Z4I
SpCas9 bound to 16-nucleotide complementary DNA substrate
Functional Information from GO Data
Chain | GOid | namespace | contents |
B | 0003677 | molecular_function | DNA binding |
B | 0003723 | molecular_function | RNA binding |
B | 0004519 | molecular_function | endonuclease activity |
B | 0004520 | molecular_function | DNA endonuclease activity |
B | 0004527 | molecular_function | exonuclease activity |
B | 0008408 | molecular_function | 3'-5' exonuclease activity |
B | 0043571 | biological_process | maintenance of CRISPR repeat elements |
B | 0046872 | molecular_function | metal ion binding |
B | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 1 |
Details | ACT_SITE: For RuvC-like nuclease domain => ECO:0000305|PubMed:24529477 |
Chain | Residue | Details |
B | ALA10 |
site_id | SWS_FT_FI2 |
Number of Residues | 1 |
Details | ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:24529477 |
Chain | Residue | Details |
B | ALA840 |
site_id | SWS_FT_FI3 |
Number of Residues | 6 |
Details | BINDING: BINDING => ECO:0000269|PubMed:24505130 |
Chain | Residue | Details |
B | ALA10 | |
B | GLU762 | |
B | GLU766 | |
B | HIS983 | |
B | HIS1297 | |
B | ASP1328 |