7Z42

Influenza B polymerase with Pol II pSer5 CTD peptide mimic bound in site 2B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Polymerase acidic protein	polymer	751	85822.8	2	UniProt (Q5V8Z9) Pfam (PF00603)	Influenza B virus
2	B, E (B, E)	RNA-directed RNA polymerase catalytic subunit	polymer	772	86207.0	2	UniProt (Q5V8Y6) Pfam (PF00602)	Influenza B virus	Polymerase basic protein 1,PB1,RNA-directed RNA polymerase subunit P1
3	C, F (C, F)	Polymerase basic protein 2	polymer	798	90844.1	2	UniProt (Q5V8X3) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952)	Influenza B virus	RNA-directed RNA polymerase subunit P3
4	G, H, J, K (Y, G, X, I)	DNA-directed RNA polymerase II subunit RPB1	polymer	28	3216.9	4	Pfam (PF05001) UniProt (by SIFTS) (P24928)	Homo sapiens
5	I, L (H, V)	RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*A)-3')	polymer	13	4212.6	2		Influenza B virus
6	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	206	Chemie (HOH)

Sequence modifications

A, D: 1 - 726 (UniProt: Q5V8Z9)

PDB	External Database	Details
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 727	-	expression tag
Ser 728	-	expression tag
Gly 729	-	expression tag
Ser 730	-	expression tag
Gly 731	-	expression tag
Glu 732	-	expression tag
Asn 733	-	expression tag
Leu 734	-	expression tag
Tyr 735	-	expression tag
Phe 736	-	expression tag
Gln 737	-	expression tag

B, E: 1 - 752 (UniProt: Q5V8Y6)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 753	-	expression tag
Ser 754	-	expression tag
Gly 755	-	expression tag
Ser 756	-	expression tag
Gly 757	-	expression tag
Glu 758	-	expression tag
Asn 759	-	expression tag
Leu 760	-	expression tag
Tyr 761	-	expression tag
Phe 762	-	expression tag
Gln 763	-	expression tag

C, F: 1 - 770 (UniProt: Q5V8X3)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 771	-	expression tag
Trp 772	-	expression tag
Ser 773	-	expression tag
His 774	-	expression tag
Pro 775	-	expression tag
Gln 776	-	expression tag
Phe 777	-	expression tag
Glu 778	-	expression tag
Lys 779	-	expression tag
Gly 780	-	expression tag
Ser 781	-	expression tag
Gly 782	-	expression tag
Ser 783	-	expression tag
Glu 784	-	expression tag
Asn 785	-	expression tag
Leu 786	-	expression tag
Tyr 787	-	expression tag
Phe 788	-	expression tag
Gln 789	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	547040.6
All*	Total formula weight	547040.6

*Water molecules are not included.