7Z42
Influenza B polymerase with Pol II pSer5 CTD peptide mimic bound in site 2B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.25422 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 130.930, 202.269, 135.733 |
Unit cell angles | 90.00, 110.54, 90.00 |
Refinement procedure
Resolution | 127.106 - 2.418 |
Rwork | 0.223 |
R-free | 0.25670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fmz |
RMSD bond length | 0.003 |
RMSD bond angle | 1.197 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 127.106 | 2.690 |
High resolution limit [Å] | 2.418 | 2.420 |
Rmerge | 0.112 | 0.618 |
Rmeas | 0.136 | 0.769 |
Number of reflections | 154519 | 7573 |
<I/σ(I)> | 7.7 | 1.9 |
Completeness [%] | 61.4 | 64 |
Redundancy | 3 | 2.5 |
CC(1/2) | 0.992 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | FluPolB PA mutant K135A at 9 mg per ml was mixed with 40 microM of nucleotides 1-13 vRNA 5prime end, 5-pAGUAGUAACAAGA-3, and 1.8 mM 28-mer CTD peptide, YSPTpSPS x 4 in a buffer containing 50 mM HEPES pH 7.5, 500 mM NaCl, 5% glycerol, 2 mM TCEP. Hanging drops for crystallization were set up at 20 C. |