7Z3X
Crystal structure of FIR RRM1-2 Y115F mutant bound to FUSE ssDNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Poly(U)-binding-splicing factor PUF60 | polymer | 197 | 21803.9 | 2 | UniProt (Q9UHX1) Pfam (PF00076) | Homo sapiens (human) | 60 kDa poly(U)-binding-splicing factor,FUSE-binding protein-interacting repressor,FBP-interacting repressor,Ro-binding protein 1,RoBP1,Siah-binding protein 1,Siah-BP1 |
| 2 | C (D) | DNA (5'-D(P*GP*TP*())-3') | polymer | 3 | 892.6 | 1 | Homo sapiens | ||
| 3 | D (E) | DNA (5'-D(*TP*TP*T)-3') | polymer | 3 | 867.6 | 1 | Homo sapiens | ||
| 4 | E, F, G, H (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 5 | I, J, K (A, B, D) | water | water | 18.0 | 177 | Chemie (HOH) |
Sequence modifications
A, B: 97 - 291 (UniProt: Q9UHX1)
| PDB | External Database | Details |
|---|---|---|
| Ser 95 | - | expression tag |
| Met 96 | - | expression tag |
| Phe 115 | Tyr 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 45368.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 248.3 | |
| All* | Total formula weight | 45616.3 |
