7Z3X

Crystal structure of FIR RRM1-2 Y115F mutant bound to FUSE ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999998
Spacegroup name	P 21 21 21
Unit cell lengths	55.613, 70.386, 107.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.380 - 1.650
R-factor	0.2044
Rwork	0.204
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qfj
RMSD bond length	0.007
RMSD bond angle	1.041
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.2-4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.380	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	50994	2538
<I/σ(I)>	16.3
Completeness [%]	99.2
Redundancy	4.3
CC(1/2)	0.999	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	25% PEG3350 0.1M Bis-Tris, pH5.5