7Z3R

Crystal structure of the mouse leptin:LepR-IgCRH2 complex to 2.95 A resolution.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Leptin	polymer	148	16220.6	1	UniProt (P41160) Pfam (PF02024)	Mus musculus (house mouse)	Obesity factor
2	B (B)	Leptin receptor	polymer	330	37429.5	1	UniProt (P48356) Pfam (PF06328) Pfam (PF18589)	Mus musculus (house mouse)	LEP-R,B219,OB receptor,OB-R
3	C (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
4	D (B)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A: 21 - 167 (UniProt: P41160)

PDB	External Database	Details
Met 20	-	initiating methionine

B: 328 - 633 (UniProt: P48356)

PDB	External Database	Details
Ala 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
Pro 311	-	expression tag
Gly 312	-	expression tag
Gly 313	-	expression tag
Pro 314	-	expression tag
Gly 315	-	expression tag
Ser 316	-	expression tag
Glu 317	-	expression tag
Asn 318	-	expression tag
Leu 319	-	expression tag
Tyr 320	-	expression tag
Phe 321	-	expression tag
Gln 322	-	expression tag
Gly 323	-	expression tag
Gly 324	-	expression tag
Ser 325	-	expression tag
Ser 326	-	expression tag
Gly 327	-	expression tag
Ser 602	Cys 602	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53650.0
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	53871.2

*Water molecules are not included.