7Z3R

Crystal structure of the mouse leptin:LepR-IgCRH2 complex to 2.95 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-31
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97620
Spacegroup name	H 3 2
Unit cell lengths	132.678, 132.678, 249.191
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.340 - 2.951
R-factor	0.2245
Rwork	0.223
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ax8
RMSD bond length	0.006
RMSD bond angle	0.860
Data reduction software	XDS (version)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.7.0)
Refinement software	BUSTER (2.10.4 (3-FEB-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.060	3.130
High resolution limit [Å]	2.950	2.950
Rmeas	0.151	3.400
Number of reflections	18075	2876
<I/σ(I)>	17.7	1.1
Completeness [%]	100.0	100
Redundancy	20	20.6
CC(1/2)	0.999	0.417

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	Proplex HT #G7 3.0 M formate 0.1 M Tris pH 7.5 cryo: 4 M Na-formate + 10% ethylene glycol