7Z2W
Escherichia coli periplasmic phytase AppA D304A,T305E mutant, complex with myo-inositol hexakissulfate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Acidphosphatase | polymer | 412 | 44871.8 | 1 | UniProt (G0ZGJ8) Pfam (PF00328) UniProt (by SIFTS) (P07102) In PDB | Escherichia coli | AppA family phytase/histidine-type acid phosphatase,Bifunctional acid phosphatase/4-phytase,Periplasmic AppA protein,Periplasmic AppA protein [includes: phosphoanhydrid phosphohydrolase and 4-phytase],Phosphoanhydride phosphohydrolase (pH 2.5 acid phosphatase) (AP) / 4- phytase,Phosphoanhydride phosphorylase,Phytase AppA |
| 2 | A | D-MYO-INOSITOL-HEXASULPHATE | non-polymer | 660.5 | 1 | Chemie (IHS) | |||
| 3 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | A | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 5 | A | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 6 | water | water | 18.0 | 360 | Chemie (HOH) |
Sequence modifications
A: 1 - 410 (UniProt: G0ZGJ8)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Ala 304 | Asp 326 | engineered mutation |
| Glu 305 | Thr 327 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 44871.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1042.8 | |
| All* | Total formula weight | 45914.7 |
