7Z0V
A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Nitrile hydratase | polymer | 207 | 23755.2 | 1 | UniProt (Q84FS5) Pfam (PF02979) | Aeribacillus pallidus | |
| 2 | B (B) | Nitrile hydratase subunit beta | polymer | 229 | 26532.1 | 1 | UniProt (Q84FS6) Pfam (PF21006) Pfam (PF02211) | Aeribacillus pallidus | NHase |
| 3 | C (A) | COBALT (II) ION | non-polymer | 58.9 | 1 | Chemie (CO) | |||
| 4 | D (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 5 | E, F (A, B) | water | water | 18.0 | 427 | Chemie (HOH) |
Sequence modifications
A: 10 - 216 (UniProt: Q84FS5)
B: 1 - 229 (UniProt: Q84FS6)
| PDB | External Database | Details |
|---|---|---|
| Arg 169 | Ser 169 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Lys 43 | Met 43 | engineered mutation |
| Ala 150 | Thr 150 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 50287.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 94.4 | |
| All* | Total formula weight | 50381.7 |
