7Z0V
A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-07-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 106.180, 106.180, 82.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.690 - 1.450 |
| R-factor | 0.1444 |
| Rwork | 0.142 |
| R-free | 0.18430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dpp |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.539 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.690 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.038 | 0.321 |
| Number of reflections | 83831 | 83831 |
| <I/σ(I)> | 13.5 | 3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 30% PEG400, 100mM magnesium chloride, 100mM MES (2[N-Morpholino]ethanesulfonic acid), 10-40mg/ml protein, pH 6.5, vapor diffusion, hanging drop, temperature 295K |
