7YW9

Crystal structure of the triple mutant CmnC-L136Q,S138G,D249Y in complex with alpha-KG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	CmnC	polymer	358	39120.6	2	UniProt (A6YEH4) Pfam (PF02668) In PDB	Saccharothrix mutabilis subsp. capreolus
2	A	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
3	A	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
4	A	D-ARGININE	non-polymer		175.2	1	Chemie (DAR)
5		water	water		18.0	430	Chemie (HOH)

Sequence modifications

A, B: 1 - 338 (UniProt: A6YEH4)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gln 136	Leu 136	engineered mutation
Gly 138	Ser 138	engineered mutation
Tyr 249	Asp 249	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78241.1
Non-Polymers*	Number of molecules	5
	Total formula weight	593.6
All*	Total formula weight	78834.8

*Water molecules are not included.