7YW9
Crystal structure of the triple mutant CmnC-L136Q,S138G,D249Y in complex with alpha-KG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2022-08-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9732 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.951, 126.671, 140.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.910 - 1.760 |
| R-factor | 0.1778 |
| Rwork | 0.177 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wbo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.052 | 0.439 |
| Number of reflections | 81412 | 8058 |
| <I/σ(I)> | 25.9 | 3.07 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20 mM ammonium acetate, 24% v/v polyethylene glycol 400, 0.1 M sodium citrate tribasic dihydrate |
