7YPZ

Zafirlukast in complex with CRM1-Ran-RanBP1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GTP-binding nuclear protein Ran	polymer	216	24400.0	1	UniProt (P62826) Pfam (PF00071)	Homo sapiens (human)	Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein
2	B (B)	YRB1 isoform 1	polymer	140	16320.7	1	UniProt (A0A6A5PZB5) Pfam (PF00638) UniProt (by SIFTS) (P41920)	Saccharomyces cerevisiae (baker's yeast)
3	C (C)	CRM1 isoform 1	polymer	1003	115224.5	1	UniProt (A0A6A5PZI8) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) UniProt (by SIFTS) (P30822)	Saccharomyces cerevisiae (baker's yeast)	HLJ1_G0022020.mRNA.1.CDS.1,Y55_G0022260.mRNA.1.CDS.1
4	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E, F, S, T, V... (A, C)	DIMETHYL SULFOXIDE	non-polymer		78.1	6	Chemie (DMS)
6	AA, G, H, I, L... (C, A)	1,2-ETHANEDIOL	non-polymer		62.1	8	Chemie (EDO)
7	J (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
8	K, M, N, O (A, C)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
9	P (C)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
10	R, U (C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
11	X (C)	3[N-MORPHOLINO]PROPANE SULFONIC ACID	non-polymer		209.3	1	Chemie (MPO)
12	BA (C)	zafirlukast	non-polymer		575.7	1	Chemie (ZLK)
13	CA, DA, EA (A, B, C)	water	water		18.0	536	Chemie (HOH)

Sequence modifications

A: 1 - 216 (UniProt: P62826)

PDB	External Database	Details
Glu 8	Gln 8	conflict
Leu 69	Gln 69	engineered mutation
Ala 182	Leu 182	engineered mutation

C: 1 - 1058 (UniProt: A0A6A5PZI8)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Glu 27	Ser 27	engineered mutation
Glu 49	Gln 49	engineered mutation
Val 51	Ala 51	engineered mutation
-	Val 377	deletion
-	Gln 378	deletion
-	Arg 379	deletion
-	Leu 380	deletion
-	Pro 381	deletion
-	Ala 382	deletion
-	Thr 383	deletion
-	Glu 384	deletion
-	Met 385	deletion
-	Ser 386	deletion
-	Pro 387	deletion
-	Leu 388	deletion
-	Ile 389	deletion
-	Gln 390	deletion
-	Leu 391	deletion
-	Ser 392	deletion
-	Val 393	deletion
-	Gly 394	deletion
-	Ser 395	deletion
-	Gln 396	deletion
-	Ala 397	deletion
-	Ile 398	deletion
-	Ser 399	deletion
-	Thr 400	deletion
-	Gly 401	deletion
-	Ser 402	deletion
-	Gly 403	deletion
-	Ala 404	deletion
-	Leu 405	deletion
-	Asn 406	deletion
-	Pro 407	deletion
-	Glu 408	deletion
-	Tyr 409	deletion
-	Met 410	deletion
-	Lys 411	deletion
-	Arg 412	deletion
-	Phe 413	deletion
-	Val 441	deletion
-	Leu 442	deletion
-	Val 443	deletion
-	Val 444	deletion
-	Glu 445	deletion
-	Asn 446	deletion
-	Asp 447	deletion
-	Glu 448	deletion
-	Gly 449	deletion
-	Glu 450	deletion
-	Ile 451	deletion
-	Val 452	deletion
-	Arg 453	deletion
-	Glu 454	deletion
-	Phe 455	deletion
-	Val 456	deletion
-	Lys 457	deletion
-	Glu 458	deletion
-	Ser 459	deletion
-	Asp 460	deletion
-	Thr 461	deletion
Gly 537	Asp 537	engineered mutation
Cys 539	Thr 539	engineered mutation
Glu 540	Val 540	engineered mutation
Gln 541	Lys 541	engineered mutation
Arg 553	Ser 553	engineered mutation
Glu 561	Gln 561	engineered mutation
Thr 741	Ala 741	engineered mutation
Cys 1022	Tyr 1022	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	155945.2
Non-Polymers*	Number of molecules	25
	Total formula weight	2646.8
All*	Total formula weight	158591.9

*Water molecules are not included.