7YK7
Cryo-EM structure of the DC591053-bound human relaxin family peptide receptor 4 (RXFP4)-Gi complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) In PDB | Bos taurus (Bovine) | G gamma-I |
2 | I | Guanine nucleotide-binding protein G(i) subunit alpha-2 | polymer | 355 | 40502.9 | 1 | UniProt (P04899) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
3 | R | Relaxin-3 receptor 2 | polymer | 374 | 41177.3 | 1 | UniProt (Q8TDU9) Pfam (PF00001) In PDB | Homo sapiens (human) | RLN3 receptor 2,G-protein coupled receptor 100,G-protein coupled receptor GPCR142,Insulin-like peptide INSL5 receptor,Relaxin family peptide receptor 4 |
4 | S | scFv16 | polymer | 248 | 26408.5 | 1 | synthetic construct | ||
5 | T | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
6 | R | [(1S)-7-ethoxy-6-methoxy-1-[2-(5-methoxy-1H-indol-3-yl)ethyl]-3,4-dihydro-1H-isoquinolin-2-yl]-morpholin-4-yl-methanone | non-polymer | 493.6 | 1 | Chemie (IYM) |
Sequence modifications
I: 1 - 355 (UniProt: P04899)
T: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Asn 47 | Ser 47 | engineered mutation |
Ala 204 | Gly 204 | engineered mutation |
Ala 246 | Glu 246 | engineered mutation |
Ser 327 | Ala 327 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 153865.3 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 493.6 | |
All* | Total formula weight | 154358.9 |