7YK7

Cryo-EM structure of the DC591053-bound human relaxin family peptide receptor 4 (RXFP4)-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
2	B (I)	Guanine nucleotide-binding protein G(i) subunit alpha-2	polymer	355	40502.9	1	UniProt (P04899) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (R)	Relaxin-3 receptor 2	polymer	374	41177.3	1	UniProt (Q8TDU9) Pfam (PF00001)	Homo sapiens (human)	RLN3 receptor 2,G-protein coupled receptor 100,G-protein coupled receptor GPCR142,Insulin-like peptide INSL5 receptor,Relaxin family peptide receptor 4
4	D (S)	scFv16	polymer	248	26408.5	1		synthetic construct
5	E (T)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
6	F (R)	[(1S)-7-ethoxy-6-methoxy-1-[2-(5-methoxy-1H-indol-3-yl)ethyl]-3,4-dihydro-1H-isoquinolin-2-yl]-morpholin-4-yl-methanone	non-polymer		493.6	1	Chemie (IYM)

Sequence modifications

I: 1 - 355 (UniProt: P04899)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 204	Gly 204	engineered mutation
Ala 246	Glu 246	engineered mutation
Ser 327	Ala 327	engineered mutation

T: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	153865.3
Non-Polymers*	Number of molecules	1
	Total formula weight	493.6
All*	Total formula weight	154358.9

*Water molecules are not included.