7YJK

Cryo-EM structure of the dimeric atSPT-ORM1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Long chain base biosynthesis protein 1	polymer	482	53207.5	2	UniProt (Q94IB8) Pfam (PF00155)	Arabidopsis thaliana (thale cress)	AtLCB1
2	B, F (B, F)	Long chain base biosynthesis protein 2a	polymer	489	54587.6	2	UniProt (Q9LSZ9) Pfam (PF00155)	Arabidopsis thaliana (thale cress)	AtLCB2a,Long chain base biosynthesis protein 2,AtLCB2
3	C, G (D, H)	ORMDL family protein	polymer	157	18220.3	2	UniProt (Q9C5I0) Pfam (PF04061)	Arabidopsis thaliana (thale cress)	atORM1
4	D, H (C, G)	Transmembrane protein, putative (DUF3317)	polymer	77	9442.7	2	UniProt (A8MSB8) Pfam (PF11779)	Arabidopsis thaliana (thale cress)	atSPTssa
5	I, J (B, F)	N-[(2S,3R,4E)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide	non-polymer		650.1	2	Chemie (Z1T)

Sequence modifications

C, G: 1 - 56 (UniProt: A8MSB8)

PDB	External Database	Details
Met -20	-	initiating methionine
Ala -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Lys -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Pro -8	-	expression tag
Asp -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	270916.0
Non-Polymers*	Number of molecules	2
	Total formula weight	1300.2
All*	Total formula weight	272216.2

*Water molecules are not included.