7YIX
The Cryo-EM Structure of Human Tissue Nonspecific Alkaline Phosphatase and Single-Chain Fragment Variable (ScFv) Complex.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Alkaline phosphatase, tissue-nonspecific isozyme | polymer | 518 | 57020.2 | 2 | UniProt (P05186) Pfam (PF00245) In PDB | Homo sapiens (human) | AP-TNAP,TNS-ALP,TNSALP,Alkaline phosphatase liver/bone/kidney isozyme,Phosphoamidase,Phosphocreatine phosphatase |
| 2 | C, D | scFv | polymer | 275 | 28830.2 | 2 | Homo sapiens (human) | ||
| 3 | E, F, G, H | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 4 | A, B | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
| 5 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | A, B | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 7 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) |
Sequence modifications
A, B: 18 - 500 (UniProt: P05186)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Lys 0 | - | expression tag |
| Thr 1 | - | expression tag |
| Ile 2 | - | expression tag |
| Ile 3 | - | expression tag |
| Ala 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Ser 6 | - | expression tag |
| Tyr 7 | - | expression tag |
| Ile 8 | - | expression tag |
| Phe 9 | - | expression tag |
| Cys 10 | - | expression tag |
| Leu 11 | - | expression tag |
| Val 12 | - | expression tag |
| Phe 13 | - | expression tag |
| Ala 14 | - | expression tag |
| Gly 15 | - | expression tag |
| Arg 16 | - | expression tag |
| Ala 17 | - | expression tag |
| Ala 501 | - | expression tag |
| Ala 502 | - | expression tag |
| Ala 503 | - | expression tag |
| Glu 504 | - | expression tag |
| Asn 505 | - | expression tag |
| Leu 506 | - | expression tag |
| Tyr 507 | - | expression tag |
| Phe 508 | - | expression tag |
| Gln 509 | - | expression tag |
| Gly 510 | - | expression tag |
| Cys 511 | - | expression tag |
| Cys 512 | - | expression tag |
| Pro 513 | - | expression tag |
| Gly 514 | - | expression tag |
| Cys 515 | - | expression tag |
| Cys 516 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 171700.8 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1697.6 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1275.2 | |
| All* | Total formula weight | 174673.6 |
