7YHP

CryoEM structure of Arabidopsis ROS1 in complex with 5mC-dsDNA at 3.1 Angstroms resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sex-determining region Y protein,REPRESSOR OF SILENCING 1,DNA glycosylase/AP lyase ROS1	polymer	757	86260.9	1	UniProt (Q05066) UniProt (Q9SJQ6) Pfam (PF00505) Pfam (PF15629) Pfam (PF15628) In PDB	Homo sapiens (human)	Testis-determining factor,DEMETER-like protein 1,Protein REPRESSOR OF SILENCING 1,Protein ROS1
2	B	DNA (40-MER)	polymer	40	12336.9	1		Arabidopsis thaliana (thale cress)
3	C	DNA (40-MER)	polymer	40	12292.9	1		Arabidopsis thaliana (thale cress)
4	A	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)

Sequence modifications

A: 431 - 505 (UniProt: Q05066)

PDB	External Database	Details
Ser 430	-	expression tag
Lys 506	-	linker
Gly 507	-	linker
Gly 508	-	linker
Ser 509	-	linker
Ser 510	-	linker

A: 511 - 835 (UniProt: Q9SJQ6)
A: 836 - 1109 (UniProt: Q9SJQ6)

PDB	External Database	Details
Asn 971	Asp 971	engineered mutation

A: 1110 - 1393 (UniProt: Q9SJQ6)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	110890.7
Non-Polymers*	Number of molecules	1
	Total formula weight	351.6
All*	Total formula weight	111242.4

*Water molecules are not included.