7YDP

Cryo-EM structure of CD97/miniGs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Adhesion G protein-coupled receptor E5 subunit beta	polymer	307	34547.0	1	UniProt (P48960) Pfam (PF00002) In PDB	Homo sapiens (human)
2	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
3	A	engineered miniGas	polymer	361	41879.5	1	Pfam (PF00503) UniProt (by SIFTS) (P63096) UniProt (by SIFTS) (P63092) In PDB	Homo sapiens
4	D	Nb35	polymer	161	17381.6	1		Lama glama
5	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1

Sequence modifications

R: 531 - 835 (UniProt: P48960)

PDB	External Database	Details
Met 530	-	initiating methionine
Leu 836	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	139584.7
All*	Total formula weight	139584.7

*Water molecules are not included.