7Y96

Crystal structure of the carboxy-terminal domain of a coronavirus M protein fused with a split GFP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein,Membrane protein	polymer	342	38118.0	2	UniProt (P42212) UniProt (A3EXD6) Pfam (PF01353) Pfam (PF01635)	Aequorea victoria	M protein,E1 glycoprotein,Matrix glycoprotein,Membrane glycoprotein

Sequence modifications

A, B: 1 - 144 (UniProt: P42212)

PDB	External Database	Details
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation

A, B: 150 - 237 (UniProt: A3EXD6)

PDB	External Database	Details
Ala 238	-	linker
Ala 239	-	linker
Ala 240	-	linker

A, B: 241 - 325 (UniProt: P42212)

PDB	External Database	Details
Val 145	-	linker
Asp 146	-	linker
Thr 147	-	linker
Met 148	-	linker
Glu 149	-	linker
Thr 248	Met 153	engineered mutation
Ala 258	Val 163	engineered mutation
Val 266	Ile 171	engineered mutation
Val 301	Ala 206	engineered mutation
Glu 326	-	expression tag
Asn 327	-	expression tag
Leu 328	-	expression tag
Tyr 329	-	expression tag
Phe 330	-	expression tag
Gln 331	-	expression tag
Gly 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag
His 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76236.0
All*	Total formula weight	76236.0

*Water molecules are not included.