7Y96

Crystal structure of the carboxy-terminal domain of a coronavirus M protein fused with a split GFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 61 2 2
Unit cell lengths	82.020, 82.020, 427.841
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.292 - 3.415
R-factor	0.2409
Rwork	0.240
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3q
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.330	46.330	3.462
High resolution limit [Å]	3.400	9.437	3.400
Rmerge	0.150	0.041	0.563
Rmeas	0.169	0.047	0.632
Rpim	0.077	0.022	0.280
Total number of observations	52103	2361	2698
Number of reflections	11907	595	596
<I/σ(I)>	8.9	23	2.7
Completeness [%]	93.1	80.2	93.9
Redundancy	4.4	4	4.5
CC(1/2)	0.990	0.997	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	293	0.4 M ammonium sulfate, 0.1 M Bis-Tris pH 5.3, PEG 3350 27%, 0.5% ethyl acetate