7Y7Y

Cryo-EM structure of human GABA transporter GAT1 bound with nipecotic acid in NaCl solution in an inward-occluded state at 2.4 angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sodium- and chloride-dependent GABA transporter 1	polymer	632	70895.9	1	UniProt (P30531) Pfam (PF00209) In PDB	Homo sapiens (human)	GAT-1,Solute carrier family 6 member 1
2	A	(3R)-piperidine-3-carboxylic acid	non-polymer		129.2	1	Chemie (ID7)
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
4	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6		water	water		18.0	54	Chemie (HOH)

Sequence modifications

A: 1 - 599 (UniProt: P30531)

PDB	External Database	Details
Leu 600	-	expression tag
Glu 601	-	expression tag
Ser 602	-	expression tag
Gly 603	-	expression tag
Asp 604	-	expression tag
Glu 605	-	expression tag
Val 606	-	expression tag
Asp 607	-	expression tag
Ala 608	-	expression tag
Ser 609	-	expression tag
Gly 610	-	expression tag
Asp 611	-	expression tag
Tyr 612	-	expression tag
Lys 613	-	expression tag
Asp 614	-	expression tag
His 615	-	expression tag
Asp 616	-	expression tag
Gly 617	-	expression tag
Asp 618	-	expression tag
Tyr 619	-	expression tag
Lys 620	-	expression tag
Asp 621	-	expression tag
His 622	-	expression tag
Asp 623	-	expression tag
Ile 624	-	expression tag
Asp 625	-	expression tag
Tyr 626	-	expression tag
Lys 627	-	expression tag
Asp 628	-	expression tag
Asp 629	-	expression tag
Asp 630	-	expression tag
Asp 631	-	expression tag
Lys 632	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	70895.9
Non-Polymers*	Number of molecules	7
	Total formula weight	874.2
All*	Total formula weight	71770.1

*Water molecules are not included.