7Y7Y

Cryo-EM structure of human GABA transporter GAT1 bound with nipecotic acid in NaCl solution in an inward-occluded state at 2.4 angstrom

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005332	molecular_function	gamma-aminobutyric acid:sodium:chloride symporter activity
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0006836	biological_process	neurotransmitter transport
A	0006865	biological_process	amino acid transport
A	0007268	biological_process	chemical synaptic transmission
A	0007612	biological_process	learning
A	0007613	biological_process	memory
A	0008306	biological_process	associative learning
A	0009636	biological_process	response to toxic substance
A	0009744	biological_process	response to sucrose
A	0009986	cellular_component	cell surface
A	0010033	biological_process	obsolete response to organic substance
A	0010243	biological_process	response to organonitrogen compound
A	0010288	biological_process	response to lead ion
A	0014054	biological_process	positive regulation of gamma-aminobutyric acid secretion
A	0014070	biological_process	response to organic cyclic compound
A	0014074	biological_process	response to purine-containing compound
A	0015185	molecular_function	gamma-aminobutyric acid transmembrane transporter activity
A	0015293	molecular_function	symporter activity
A	0015378	molecular_function	sodium:chloride symporter activity
A	0016020	cellular_component	membrane
A	0022857	molecular_function	transmembrane transporter activity
A	0030424	cellular_component	axon
A	0032229	biological_process	negative regulation of synaptic transmission, GABAergic
A	0032355	biological_process	response to estradiol
A	0035725	biological_process	sodium ion transmembrane transport
A	0042220	biological_process	response to cocaine
A	0042734	cellular_component	presynaptic membrane
A	0042802	molecular_function	identical protein binding
A	0042995	cellular_component	cell projection
A	0043025	cellular_component	neuronal cell body
A	0045202	cellular_component	synapse
A	0045211	cellular_component	postsynaptic membrane
A	0046872	molecular_function	metal ion binding
A	0050808	biological_process	synapse organization
A	0051592	biological_process	response to calcium ion
A	0051936	biological_process	gamma-aminobutyric acid reuptake
A	0051939	biological_process	gamma-aminobutyric acid import
A	0098658	biological_process	inorganic anion import across plasma membrane
A	0098719	biological_process	sodium ion import across plasma membrane
A	0098793	cellular_component	presynapse
A	0098810	biological_process	neurotransmitter reuptake
A	0098982	cellular_component	GABA-ergic synapse
A	0150104	biological_process	transport across blood-brain barrier
A	1902476	biological_process	chloride transmembrane transport

Functional Information from PROSITE/UniProt

site_id	PS00610
Number of Residues	15
Details	NA_NEUROTRAN_SYMP_1 Sodium:neurotransmitter symporter family signature 1. WRFPYlcgkNGGGaF

Chain	Residue	Details
A	TRP68-PHE82

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site_id	PS00754
Number of Residues	21
Details	NA_NEUROTRAN_SYMP_2 Sodium:neurotransmitter symporter family signature 2. YLynSFTttLPWkqCdnpwNT

Chain	Residue	Details
A	TYR150-THR170

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	179
Details	TOPO_DOM: Cytoplasmic => ECO:0000250|UniProtKB:P23978

Chain	Residue	Details
A	MET1-ASP52
A	GLU101-LYS123
A	LYS231-LYS238
A	ASN310-SER320
A	ASP395-GLU421
A	ASN478-LYS497
A	MET555-ILE599

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	TRANSMEM: Helical; Name=1 => ECO:0000255

Chain	Residue	Details
A	PHE53-LEU73

---

site_id	SWS_FT_FI3
Number of Residues	169
Details	TOPO_DOM: Extracellular => ECO:0000250|UniProtKB:P23978

Chain	Residue	Details
A	CYS74-GLY80
A	SER145-ARG211
A	ARG257-GLN291
A	GLY343-GLN374
A	GLN441-SER456
A	GLN518-GLY535

---

site_id	SWS_FT_FI4
Number of Residues	19
Details	TRANSMEM: Helical; Name=2 => ECO:0000255

Chain	Residue	Details
A	ALA81-LEU100

---

site_id	SWS_FT_FI5
Number of Residues	20
Details	TRANSMEM: Helical; Name=3 => ECO:0000255

Chain	Residue	Details
A	GLY124-ILE144

---

site_id	SWS_FT_FI6
Number of Residues	18
Details	TRANSMEM: Helical; Name=4 => ECO:0000255

Chain	Residue	Details
A	TRP212-TRP230

---

site_id	SWS_FT_FI7
Number of Residues	17
Details	TRANSMEM: Helical; Name=5 => ECO:0000255

Chain	Residue	Details
A	VAL239-PHE256

---

site_id	SWS_FT_FI8
Number of Residues	17
Details	TRANSMEM: Helical; Name=6 => ECO:0000255

Chain	Residue	Details
A	ILE292-TYR309

---

site_id	SWS_FT_FI9
Number of Residues	21
Details	TRANSMEM: Helical; Name=7 => ECO:0000255

Chain	Residue	Details
A	ILE321-VAL342

---

site_id	SWS_FT_FI10
Number of Residues	19
Details	TRANSMEM: Helical; Name=8 => ECO:0000255

Chain	Residue	Details
A	LEU375-ILE394

---

site_id	SWS_FT_FI11
Number of Residues	18
Details	TRANSMEM: Helical; Name=9 => ECO:0000255

Chain	Residue	Details
A	LEU422-THR440

---

site_id	SWS_FT_FI12
Number of Residues	20
Details	TRANSMEM: Helical; Name=10 => ECO:0000255

Chain	Residue	Details
A	GLY457-VAL477

---

site_id	SWS_FT_FI13
Number of Residues	19
Details	TRANSMEM: Helical; Name=11 => ECO:0000255

Chain	Residue	Details
A	LEU498-VAL517

---

site_id	SWS_FT_FI14
Number of Residues	18
Details	TRANSMEM: Helical; Name=12 => ECO:0000255

Chain	Residue	Details
A	VAL536-TYR554

---

site_id	SWS_FT_FI15
Number of Residues	9
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q7K4Y6

Chain	Residue	Details
A	GLY59
A	ALA61
A	ILE62
A	ASN66
A	SER295
A	ASN327
A	LEU392
A	ASP395
A	SER396

---

site_id	SWS_FT_FI16
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P31648

Chain	Residue	Details
A	SER18
A	SER591

---

site_id	SWS_FT_FI17
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
A	ASN176
A	ASN181
A	ASN184

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