7Y6U

Symmetry-expanded and locally refined protomer structure of IPEC-J2 cell-derived PEDV PT52 S with a CTD-close conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Spike glycoprotein	polymer	1402	153739.6	1	UniProt (A0A1Y0DD46)	Porcine epidemic diarrhea virus	S glycoprotein,E2,Peplomer protein
2	B, L (B, L)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	2	In PDB GlyTouCan (G32152BH)
3	C (C)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
4	D, G (D, G)	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	2	In PDB GlyTouCan (G86851RC)
5	E, H, I, K (E, H, I, K)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)
6	F, J (F, J)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
7	M, N, O, P, Q... (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)

Sequence modifications

A: 1 - 1327 (UniProt: A0A1Y0DD46)

PDB	External Database	Details
Pro 1076	Ile 1076	engineered mutation
Pro 1077	Leu 1077	engineered mutation
Glu 1328	-	expression tag
Phe 1329	-	expression tag
Gly 1330	-	expression tag
Ser 1331	-	expression tag
Gly 1332	-	expression tag
Gly 1333	-	expression tag
Tyr 1334	-	expression tag
Ile 1335	-	expression tag
Pro 1336	-	expression tag
Glu 1337	-	expression tag
Ala 1338	-	expression tag
Pro 1339	-	expression tag
Arg 1340	-	expression tag
Asp 1341	-	expression tag
Gly 1342	-	expression tag
Gln 1343	-	expression tag
Ala 1344	-	expression tag
Tyr 1345	-	expression tag
Val 1346	-	expression tag
Arg 1347	-	expression tag
Lys 1348	-	expression tag
Asp 1349	-	expression tag
Gly 1350	-	expression tag
Glu 1351	-	expression tag
Trp 1352	-	expression tag
Val 1353	-	expression tag
Leu 1354	-	expression tag
Leu 1355	-	expression tag
Ser 1356	-	expression tag
Thr 1357	-	expression tag
Phe 1358	-	expression tag
Leu 1359	-	expression tag
Lys 1360	-	expression tag
Gly 1361	-	expression tag
Gln 1362	-	expression tag
Asp 1363	-	expression tag
Asn 1364	-	expression tag
Ser 1365	-	expression tag
Ala 1366	-	expression tag
Asp 1367	-	expression tag
Ile 1368	-	expression tag
Gln 1369	-	expression tag
His 1370	-	expression tag
Ser 1371	-	expression tag
Gly 1372	-	expression tag
Arg 1373	-	expression tag
Pro 1374	-	expression tag
Leu 1375	-	expression tag
Glu 1376	-	expression tag
Ser 1377	-	expression tag
Arg 1378	-	expression tag
Gly 1379	-	expression tag
Pro 1380	-	expression tag
Phe 1381	-	expression tag
Glu 1382	-	expression tag
Gln 1383	-	expression tag
Lys 1384	-	expression tag
Leu 1385	-	expression tag
Ile 1386	-	expression tag
Ser 1387	-	expression tag
Glu 1388	-	expression tag
Glu 1389	-	expression tag
Asp 1390	-	expression tag
Leu 1391	-	expression tag
Asn 1392	-	expression tag
Met 1393	-	expression tag
His 1394	-	expression tag
Thr 1395	-	expression tag
Gly 1396	-	expression tag
His 1397	-	expression tag
His 1398	-	expression tag
His 1399	-	expression tag
His 1400	-	expression tag
His 1401	-	expression tag
His 1402	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	153739.6
Branched	Number of molecules	11
	Total formula weight	6305.9
Non-Polymers*	Number of molecules	7
	Total formula weight	1548.5
All*	Total formula weight	161593.9

*Water molecules are not included.