7Y6I
Cryo-EM structure of human sodium-chloride cotransporter
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Solute carrier family 12 member 3 | polymer | 1053 | 116921.5 | 2 | UniProt (P55017) Pfam (PF08403) Pfam (PF00324) Pfam (PF03522) | Homo sapiens (human) | Na-Cl cotransporter, cation-chloride cotransporter |
| 2 | C, D (C, D) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 3 | E, G, X, Z (A, B) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) PubChem (312) | |||
| 4 | AA, F, H, Y (B, A) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) PubChem (923) | |||
| 5 | BA, CA, DA, I, J... (B, A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 6 | Chemie (Y01) PubChem (65082) PubChem (363416) PubChem (500055) PubChem (13301926) PubChem (13301927) PubChem (16219150) PubChem (22210610) PubChem (45489984) PubChem (57163942) PubChem (89891013) PubChem (99567468) PubChem (124427194) PubChem (124897021) PubChem (124897020) PubChem (10005657) PubChem (130340716) PubChem (11969853) PubChem (137392566) PubChem (137496323) PubChem (155804219) PubChem (169132514) PubChem (60078761) PubChem (73440805) PubChem (99567469) PubChem (124301053) PubChem (124302591) PubChem (124303794) PubChem (124421343) PubChem (124427195) PubChem (124914595) PubChem (124914596) PubChem (131705526) PubChem (137332121) PubChem (137496357) PubChem (146157332) PubChem (154929912) PubChem (155199678) PubChem (156327905) PubChem (156426247) PubChem (156516588) PubChem (166979870) PubChem (167024614) PubChem (169132513) PubChem (170117245) PubChem (172400028) | |||
| 6 | EA, FA, GA, HA, L... (B, A) | 1-O-OCTADECYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 510.7 | 8 | Chemie (LPE) PubChem (3855) PubChem (9546711) PubChem (446876) | |||
| 7 | IA, JA, P, Q, R... (B, A) | (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate | non-polymer | 760.1 | 9 | Chemie (POV) PubChem (65167) PubChem (5497103) PubChem (6436017) PubChem (6506401) PubChem (10908846) PubChem (168614) PubChem (6070971) | |||
| 8 | KA, U (B, A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 9 | LA, MA (A, B) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 1021 (UniProt: P55017)
| PDB | External Database | Details |
|---|---|---|
| - | Gln 95 | deletion |
| Gly 264 | Ala 264 | engineered mutation |
| Leu 1022 | - | expression tag |
| Glu 1023 | - | expression tag |
| Gly 1024 | - | expression tag |
| Ser 1025 | - | expression tag |
| Asp 1026 | - | expression tag |
| Glu 1027 | - | expression tag |
| Val 1028 | - | expression tag |
| Asp 1029 | - | expression tag |
| Ala 1030 | - | expression tag |
| Gly 1031 | - | expression tag |
| Ser 1032 | - | expression tag |
| His 1033 | - | expression tag |
| His 1034 | - | expression tag |
| His 1035 | - | expression tag |
| His 1036 | - | expression tag |
| His 1037 | - | expression tag |
| His 1038 | - | expression tag |
| His 1039 | - | expression tag |
| His 1040 | - | expression tag |
| His 1041 | - | expression tag |
| His 1042 | - | expression tag |
| Gly 1043 | - | expression tag |
| Ser 1044 | - | expression tag |
| Val 1045 | - | expression tag |
| Glu 1046 | - | expression tag |
| Asp 1047 | - | expression tag |
| Tyr 1048 | - | expression tag |
| Lys 1049 | - | expression tag |
| Asp 1050 | - | expression tag |
| Asp 1051 | - | expression tag |
| Asp 1052 | - | expression tag |
| Asp 1053 | - | expression tag |
| Lys 1054 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 233842.9 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 848.8 | |
| Non-Polymers* | Number of molecules | 33 |
| Total formula weight | 14522.9 | |
| All* | Total formula weight | 249214.6 |
