7Y6I
Cryo-EM structure of human sodium-chloride cotransporter
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Solute carrier family 12 member 3 | polymer | 1053 | 116921.5 | 2 | UniProt (P55017) Pfam (PF08403) Pfam (PF00324) Pfam (PF03522) | Homo sapiens (human) | Na-Cl cotransporter, cation-chloride cotransporter |
| 2 | C, D (C, D) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 3 | E, G, X, Z (A, B) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 4 | AA, F, H, Y (B, A) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
| 5 | BA, CA, DA, I, J... (B, A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 6 | Chemie (Y01) | |||
| 6 | EA, FA, GA, HA, L... (B, A) | 1-O-OCTADECYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 510.7 | 8 | Chemie (LPE) | |||
| 7 | IA, JA, P, Q, R... (B, A) | (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate | non-polymer | 760.1 | 9 | Chemie (POV) | |||
| 8 | KA, U (B, A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 9 | LA, MA (A, B) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 1021 (UniProt: P55017)
| PDB | External Database | Details |
|---|---|---|
| - | Gln 95 | deletion |
| Gly 264 | Ala 264 | engineered mutation |
| Leu 1022 | - | expression tag |
| Glu 1023 | - | expression tag |
| Gly 1024 | - | expression tag |
| Ser 1025 | - | expression tag |
| Asp 1026 | - | expression tag |
| Glu 1027 | - | expression tag |
| Val 1028 | - | expression tag |
| Asp 1029 | - | expression tag |
| Ala 1030 | - | expression tag |
| Gly 1031 | - | expression tag |
| Ser 1032 | - | expression tag |
| His 1033 | - | expression tag |
| His 1034 | - | expression tag |
| His 1035 | - | expression tag |
| His 1036 | - | expression tag |
| His 1037 | - | expression tag |
| His 1038 | - | expression tag |
| His 1039 | - | expression tag |
| His 1040 | - | expression tag |
| His 1041 | - | expression tag |
| His 1042 | - | expression tag |
| Gly 1043 | - | expression tag |
| Ser 1044 | - | expression tag |
| Val 1045 | - | expression tag |
| Glu 1046 | - | expression tag |
| Asp 1047 | - | expression tag |
| Tyr 1048 | - | expression tag |
| Lys 1049 | - | expression tag |
| Asp 1050 | - | expression tag |
| Asp 1051 | - | expression tag |
| Asp 1052 | - | expression tag |
| Asp 1053 | - | expression tag |
| Lys 1054 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 233842.9 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 848.8 | |
| Non-Polymers* | Number of molecules | 33 |
| Total formula weight | 14522.9 | |
| All* | Total formula weight | 249214.6 |
