Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7Y6I

Cryo-EM structure of human sodium-chloride cotransporter

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006811	biological_process	monoatomic ion transport
A	0006814	biological_process	sodium ion transport
A	0006884	biological_process	cell volume homeostasis
A	0008511	molecular_function	sodium:potassium:chloride symporter activity
A	0015081	molecular_function	sodium ion transmembrane transporter activity
A	0015293	molecular_function	symporter activity
A	0015377	molecular_function	chloride:monoatomic cation symporter activity
A	0015378	molecular_function	sodium:chloride symporter activity
A	0016020	cellular_component	membrane
A	0016324	cellular_component	apical plasma membrane
A	0022857	molecular_function	transmembrane transporter activity
A	0035725	biological_process	sodium ion transmembrane transport
A	0055064	biological_process	chloride ion homeostasis
A	0055075	biological_process	potassium ion homeostasis
A	0055078	biological_process	sodium ion homeostasis
A	0055085	biological_process	transmembrane transport
A	0070062	cellular_component	extracellular exosome
A	0070294	biological_process	renal sodium ion absorption
A	1902074	biological_process	response to salt
A	1902476	biological_process	chloride transmembrane transport
A	1904044	biological_process	response to aldosterone
A	1990573	biological_process	potassium ion import across plasma membrane
B	0000166	molecular_function	nucleotide binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006811	biological_process	monoatomic ion transport
B	0006814	biological_process	sodium ion transport
B	0006884	biological_process	cell volume homeostasis
B	0008511	molecular_function	sodium:potassium:chloride symporter activity
B	0015081	molecular_function	sodium ion transmembrane transporter activity
B	0015293	molecular_function	symporter activity
B	0015377	molecular_function	chloride:monoatomic cation symporter activity
B	0015378	molecular_function	sodium:chloride symporter activity
B	0016020	cellular_component	membrane
B	0016324	cellular_component	apical plasma membrane
B	0022857	molecular_function	transmembrane transporter activity
B	0035725	biological_process	sodium ion transmembrane transport
B	0055064	biological_process	chloride ion homeostasis
B	0055075	biological_process	potassium ion homeostasis
B	0055078	biological_process	sodium ion homeostasis
B	0055085	biological_process	transmembrane transport
B	0070062	cellular_component	extracellular exosome
B	0070294	biological_process	renal sodium ion absorption
B	1902074	biological_process	response to salt
B	1902476	biological_process	chloride transmembrane transport
B	1904044	biological_process	response to aldosterone
B	1990573	biological_process	potassium ion import across plasma membrane

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	58
Details	Transmembrane: {"description":"Discontinuously helical; Name=1","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	42
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	182
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	44
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	148
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	48
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	44
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	68
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	42
Details	Transmembrane: {"description":"Discontinuously helical; Name=6","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	42
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	46
Details	Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	28
Details	Transmembrane: {"description":"Helical; Name=9","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	32
Details	Transmembrane: {"description":"Helical; Name=10","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	38
Details	Transmembrane: {"description":"Helical; Name=11","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	22
Details	Transmembrane: {"description":"Helical; Name=12","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHN","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI23
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)