7Y36
Cryo-EM structure of the Teriparatide-bound human PTH1R-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Parathyroid hormone/parathyroid hormone-related peptide receptor | polymer | 727 | 81730.6 | 1 | UniProt (Q03431) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (human) | PTH/PTHrP type I receptor,PTH/PTHr receptor,Parathyroid hormone 1 receptor,PTH1 receptor |
| 2 | B (P) | Parathyroid hormone | polymer | 34 | 4125.8 | 1 | UniProt (P01270) Pfam (PF01279) | Homo sapiens | Teriparatide,PTH,Parathormone,Parathyrin |
| 3 | C (A) | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 380 | 44257.1 | 1 | UniProt (P63092-2) Pfam (PF00503) UniProt (by SIFTS) (P63092) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 380 | 41441.3 | 1 | UniProt (P54311) Pfam (PF00400) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (cattle) | G gamma-I |
| 6 | F (N) | NanoBody 35 | polymer | 126 | 13711.3 | 1 | synthetic construct |
Sequence modifications
R: 27 - 593 (UniProt: Q03431)
R: 594 - 753 (PDB: 7Y36)
A: 1 - 394 (UniProt: P63092-2)
B: 2 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| Ala 188 | Gly 188 | conflict |
A: 1 - 394 (UniProt: P63092-2)
| PDB | External Database | Details |
|---|---|---|
| Ala 226 | Gly 212 | engineered mutation |
| Ala 268 | Glu 254 | engineered mutation |
| Lys 271 | Asn 257 | engineered mutation |
| Asp 274 | Lys 260 | engineered mutation |
| Lys 280 | Arg 266 | engineered mutation |
| Asp 284 | Thr 270 | engineered mutation |
| Thr 285 | Ile 271 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Gly 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Ser 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Gly 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Gly 352 | - | expression tag |
| Ser 353 | - | expression tag |
| Ser 354 | - | expression tag |
| Gly 355 | - | expression tag |
| Val 356 | - | expression tag |
| Ser 357 | - | expression tag |
| Gly 358 | - | expression tag |
| Trp 359 | - | expression tag |
| Arg 360 | - | expression tag |
| Leu 361 | - | expression tag |
| Phe 362 | - | expression tag |
| Lys 363 | - | expression tag |
| Lys 364 | - | expression tag |
| Ile 365 | - | expression tag |
| Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 192996.0 | |
| All* | Total formula weight | 192996.0 |
