7Y14

Cryo-EM structure of MrgD-Gi complex with beta-alanine (local)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Soluble cytochrome b562,Mas-related G-protein coupled receptor member D	polymer	446	50141.3	1	UniProt (P0ABE7) UniProt (Q8TDS7) Pfam (PF07361) Pfam (PF00001) In PDB	Escherichia coli	Cytochrome b-562,Beta-alanine receptor,G-protein coupled receptor TGR7
2	R	BETA-ALANINE	non-polymer		89.1	1	Chemie (BAL)
3	R	PALMITIC ACID	non-polymer		256.4	4	Chemie (PLM)

Sequence modifications

R: -100 - 4 (UniProt: P0ABE7)

PDB	External Database	Details
Met -124	-	initiating methionine
Lys -123	-	expression tag
Thr -122	-	expression tag
Ile -121	-	expression tag
Ile -120	-	expression tag
Ala -119	-	expression tag
Leu -118	-	expression tag
Ser -117	-	expression tag
Tyr -116	-	expression tag
Ile -115	-	expression tag
Phe -114	-	expression tag
Cys -113	-	expression tag
Leu -112	-	expression tag
Val -111	-	expression tag
Phe -110	-	expression tag
Ala -109	-	expression tag
Asp -108	-	expression tag
Tyr -107	-	expression tag
Lys -106	-	expression tag
Asp -105	-	expression tag
Asp -104	-	expression tag
Asp -103	-	expression tag
Asp -102	-	expression tag
Lys -101	-	expression tag
Trp -94	Met 29	engineered mutation
Ile 1	His 124	engineered mutation

R: 5 - 321 (UniProt: Q8TDS7)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50141.3
Non-Polymers*	Number of molecules	5
	Total formula weight	1114.8
All*	Total formula weight	51256.1

*Water molecules are not included.