7XXP

F316A-glycine-streptothricin F complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	N-formimidoyl fortimicin A synthase	polymer	508	54424.2	8	UniProt (A0A125SZC1) Pfam (PF01266) In PDB	Streptomyces luteocolor
2	G, H, A, B, C...	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	8	Chemie (FAD)
3	G, H, A, B, C...	GLYCINE	non-polymer		75.1	8	Chemie (GLY)
4	G, H, A, B, C...	Streptothricin F	non-polymer		502.5	8	Chemie (OI9)
5		water	water		18.0	1827	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 491 (UniProt: A0A125SZC1)

PDB	External Database	Details
Met -16	-	initiating methionine
Gly -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Ala 316	Phe 316	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	435393.9
Non-Polymers*	Number of molecules	24
	Total formula weight	10905.1
All*	Total formula weight	446299.0

*Water molecules are not included.