7XXP
F316A-glycine-streptothricin F complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2022-05-18 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 103.310, 106.404, 135.541 |
Unit cell angles | 90.01, 90.01, 96.21 |
Refinement procedure
Resolution | 29.919 - 2.052 |
Rwork | 0.188 |
R-free | 0.20820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xqa |
RMSD bond length | 0.008 |
RMSD bond angle | 1.488 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.081 | 0.617 |
Rmeas | 0.093 | 0.712 |
Rpim | 0.045 | 0.352 |
Number of reflections | 352119 | 34675 |
<I/σ(I)> | 17.5 | 2.4 |
Completeness [%] | 98.2 | 96.8 |
Redundancy | 4.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH8.5, 0.2 M KBr, 8 % PEG 550 MME and 8 % PEG 20000 |